This work is devoted to the substantiation and selection of patients with ovarian tumor for the purpose of conducting expensive molecular genetic studies on genotyping. As diagnostic methods have been used ultraviolet spectrometry samples of blood plasma in the liquid state, infrared spectroscopy middle range (2,5 - 25 microns) dry residue of plasma polarization and laser diagnostic technique of thin histological sections of biological tissues. Obtained results showed that the use of spectrophotometry in the range of 1000-3000 cm-1 allowed to establish quantitative parameters of the plasma absorption rate of blood of patients in the third group in different ranges, which would allow in the future to conduct an express analysis of the patient's condition (procedure screening) for further molecular-genetic typing on BRCA I and II.
The purpose of the study: to conduct laser-spectrophotometric differential diagnosis of blood in postmenopausal patients with benign processes and endometrial cancer. It was analysed on this work the dependings of autocorrelation functionsK[S ( x)] i=2;3;4 ∆ of coordinate distributions of the Stokes vector parameters polarization-inhomogeneous laser object fields of eksocervix native smears and cervical canal wall. It was revealed the behavior of power spectra distribution parameters Stokes vector ( , ) 1,2,3,4 S x y i= of object field cervix. Choice of statistical studies set points 1-4 orders polarized coherent radiation field, transformed as a result of interaction with the oncologic modified biological layers "epithelium - stroma" as a quantitative criterion polarimetric optical differentiation state of human biological tissues was proved. It is established that the increase in the value of birefringence layers of epithelial dysplasia in biological tissues and networks of collagen fibers with adenocarcinoma manifested in the growth of statistical significance since the 3rd order coordinate distributions of polarization of laser images smears and tissue sections cervix.
The purpose of the work is to demonstrate the effectiveness of optical diagnostics of cytological smears of the cervix for the presence of HPV using spectral-polarization methods. 30 cytological smears of the cervix with koilocytosis and 15 samples without koilocytosis were studied. Comparison of the obtained results with the viral load of HPV genomic equivalents in a smear-scraping by the PCR method, it was established that at wavelengths of 405-425 nm, less than 3 HE and 100 thousand cells correspond (clinically insignificant result). In contrast, at wavelengths of 425-440 nm corresponds to more than 5 HE per 100 thousand cells, which is a clinically significant variant (high carcinogenic risk). On the basis of the conducted research, it was established that the use of spectro-polarization research and the fluorescence method allows to increase the accuracy of the selection of patients for an expensive procedure - DNA diagnosis of HPV with a high carcinogenic risk by the standard PCR method.
The purpose of the work was to demonstrate the possibility of optical diagnosis in the consideration of sexually transmitted infections in the development of inflammatory processes of the cervix using spectral and polarization methods. Comparison of cervical specimens with and without coilocytosis, irradiated with a range-shifted polarized radiation, showed significant differences in the values of linear dichroism and its spectral dependences. The difference between the coilocyte response characteristic in the range 400-420 nm was calculated using computer programs. As a result, the use of spectropolarization studies and the fluorescence method will improve the accuracy of patient selection for a costly procedure - high-carcinogenic DNA diagnostics of HPV by the standard method of polymerase chain reaction.
The aim of this work is to improve the diagnosis of cervical cancer by introducing laser polarimetry and spectro polarization methods of investigation. We have proposed a novel approach for the differentiation of squamous cell carcinoma and cervical adenocarcinoma using laser optics. Obtained by the method of laser polarimetry by Stokes parameter S4 of native smear with adenocarcinoma of the cervix and squamous cell carcinoma, as well as scraping of the mud canal with endometrial adenocarcinoma and flat cell cancer allows to reliably differentiate the norm from cancer in the native smear and adenocarcinoma from the flat cell cancer in the smear-imprint. The method of spectropolarimetry allows reliably accurately distinguishing the normal epithelium of the cervix from cancer of the cervix, and the parameters of linear dichroism during the spectropolarization study, reliably (р=0,001) differentiate between normal, adenocarcinoma and flat cell cancer of the cervix.
The purpose of the work was to demonstrate the possibility of optical diagnosis of cytological smears of the cervix for the presence of HPV using spectral and polarization methods. Comparison of cervical specimens with and without coilocytosis, irradiated with a range-shifted polarized radiation, showed significant differences in the values of linear dichroism and its spectral dependences. The difference between the coilocyte response characteristic in the range 395-415 nm was calculated using computer programs. As a result, the use of spectropolarization studies and the fluorescence method will improve the accuracy of patient selection for a costly procedure - high-carcinogenic DNA diagnostics of HPV by the standard method of polymerase chain reaction.
The aim of this work is to improve the diagnosis of cervical cancer by introducing laser polarimetry and spectropolarization methods of investigation. We have proposed a novel approach for the differentiation of squamous cell carcinoma and cervical adenocarcinoma using laser optics. Obtained by the method of laser polarimetry by Stokes parameter S4 of native smear with adenocarcinoma of the cervix and squamous cell carcinoma, as well as scraping of the mud canal with endometrial adenocarcinoma and flat cell cancer allows to reliably differentiate the norm from cancer in the native smear and adenocarcinoma from the flat cell cancer in the smear-imprint. The method of spectropolarimetry allows reliably accurately distinguishing the normal epithelium of the cervix from cancer of the cervix, and the parameters of linear dichroism during the spectropolarization study, reliably (р=0,001) differentiate between normal, adenocarcinoma and flat cell cancer of the cervix.
The aim of this paper is to study BRCA1 - 185delAg and 5382insC gene mutations in breast cancer patients and their relatives to identify new directions for the prevention, diagnosis and treatment of breast cancer. As diagnostic methods have been used ultraviolet spectrometry samples of blood plasma in the liquid state, infrared spectroscopy middle range (2,5 - 25 microns) dry residue of plasma polarization and laser diagnostic technique of thin histological sections of biological tissues. Obtained results showed that the use of spectrophotometry in the range of 1000-3000 cm-1 allowed to establish quantitative parameters of the plasma absorption rate of blood of patients in the third group in different ranges, which would allow in the future to conduct an express analysis of the patient's condition (procedure screening) for further molecular-genetic typing on BRCA I and II.
The aim of this paper is to study BRCA1 - 185delAg and 5382insC gene mutations in breast cancer patients and their relatives to identify new directions for the prevention, diagnosis and treatment of breast cancer. As diagnostic methods have been used ultraviolet spectrometry samples of blood plasma in the liquid state, infrared spectroscopy middle range (2,5 - 25 microns) dry residue of plasma polarization and laser diagnostic technique of thin histological sections of biological tissues. Obtained results showed that the use of spectrophotometry in the range of 1000-3000 cm-1 allowed to establish quantitative parameters of the plasma absorption rate of blood of patients in the third group in different ranges, which would allow in the future to conduct an express analysis of the patient's condition (procedure screening) for further molecular-genetic typing on BRCA I and II.
The aim of this work is to improve the diagnosis of cervical cancer by introducing laser polarimetry and spectropolarization methods of investigation. We have proposed a novel approach for the differentiation of squamous cell carcinoma and cervical adenocarcinoma using laser optics. Obtained by the method of laser polarimetry by Stokes parameter S4 of native smear with adenocarcinoma of the cervix and squamous cell carcinoma, as well as scraping of the mud canal with endometrial adenocarcinoma and flat cell cancer allows to reliably differentiate the norm from cancer in the native smear and adenocarcinoma from the flat cell cancer in the smear-imprint. The method of spectropolarimetry allows reliably accurately distinguishing the normal epithelium of the cervix from cancer of the cervix, and the parameters of linear dichroism during the spectropolarization study, reliably (р=0,001) differentiate between normal, adenocarcinoma and flat cell cancer of the cervix.
The purpose of the work was to demonstrate the possibility of optical diagnosis of cytological smears of the cervix for the presence of HPV using spectral and polarization methods. Comparison of cervical specimens with and without coilocytosis, irradiated with a range-shifted polarized radiation, showed significant differences in the values of linear dichroism and its spectral dependences. The difference between the coilocyte response characteristic in the range 395-415 nm was calculated using computer programs. As a result, the use of spectropolarization studies and the fluorescence method will improve the accuracy of patient selection for a costly procedure - high-carcinogenic DNA diagnostics of HPV by the standard method of polymerase chain reaction.
The aim of this study was to use the spectrophotometry method to develop a diagnostic algorithm for blood studies and the content of douglas deepening in women with ovarian tumors. A comparative analysis of the blood of healthy women and patients with ovarian cancer revealed significantly greater optical anisotropy of the latter. Qualitative studies of polarization microscopic blood images revealed a very developed microcrystalline structure. Based on the study of blood and puncture and douglas deepening of healthy women and patients with benign and malignant tumors of the ovaries, using the method of laser polarimetry, experimentally developed and clinically tested photometric and polarization criteria indicating the presence of malignancy of the tumor.
The purpose of the study was to determine the histochemical and laser criteria for diagnosis of background, precancerous and endometrial cancer by the state of the cervical canal wall. The given data on the state of connective tissue in the endocervix can distinguish three differential prognostic possibilities: 1) prediction of the condition of the connective tissue of the endocervix of the normal endometrium without the possibility of differentiating the phases of the ovarian cycle; 2) prediction of the endocervix endotracheal connective tissue state of the endometrium as a separate process; 3) prediction for the condition of the connective tissue of the endocervix of the processes of expressed proliferation of the typical (glandular hyperplasia and glandular polyps) or atypical (adenocarcinoma) glandular first endometrial epithelial differentiation without the possibility of these processes among them. The stroke-scrape of the epithelium of the cervical canal (endocervix) allows the condition of the connective tissue to diagnose the processes of pronounced proliferation of the typical (hyperplasia, polyp) and atypical (adenocarcinoma) epithelium of the endometrium without the possibility of differentiating these processes among themselves.
This work is devoted to the substantiation and selection of patients with breast cancer (BC) for the purpose of conducting expensive molecular genetic studies on genotyping. As diagnostic methods have been used ultraviolet spectrometry samples of blood plasma in the liquid state, infrared spectroscopy middle range (2,5 - 25 microns) dry residue of plasma polarization and laser diagnostic technique of thin histological sections of biological tissues. Obtained results showed that the use of spectrophotometry in the range of 1000-3000 cm-1 allowed to establish quantitative parameters of the plasma absorption rate of blood of patients in the third group in different ranges, which would allow in the future to conduct an express analysis of the patient's condition (procedure screening) for further molecular-genetic typing on BRCA I and II.
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