The results of experimental studies of coordinate distributions of Mueller matrix elements of the following types of cervical
scraping tissue are presented: rate- low-grade - highly differentiated dysplasia (CIN1-CIN3) - adenocarcinoma of high, medium
and low levels of differentiation (G1-G3). The rationale for the choice of statistical points 1-4 orders polarized coherent
radiation field, transformed as a result of interaction with the oncologic modified biological layers "epithelium-stroma"
as a quantitative criterion of polarimetric optical differentiation state of human biological tissues are shown here. The
analysis of the obtained Mueller matrix elements and statistical correlation methods, the systematized by types studied
tissues is accomplished. The results of research images of Mueller matrix elements m34 for this type of pathology as low-grade
dysplasia (CIN2), the results of its statistical and correlation analysis are presented.
The aim of the study is combining polarimetry and spectropolarimetry techniques for identifying the changes of opticalgeometrical structure in different kinds of biotissues with solid tumours. It is researched that a linear dichroism appears in biotissues (human esophagus, muscle tissue of rats, human prostate tissue, cervical smear) with cancer diseases, magnitude of which depends on the type of the tissue and on the time of cancer process development.
The aim was to study the possibility of using polarimetry methods of performance evaluation of blood plasma of patients with breast cancer and spectroscopy method in the diagnosis of breast cancer and determine the criteria for their use of non-invasive screening for problems.
We studied a methods of assessment of a connective tissue of cervix in terms of specific volume of fibrous component
and an optical density of staining of connective tissue fibers in the stroma of squamous cancer and cervix
adenocarcinoma. An absorption spectra of blood plasma of the patients suffering from squamous cancer and cervix
adenocarcinoma both before the surgery and in postsurgical periods were obtained. Linear dichroism measurements
transmittance in polarized light at different orientations of the polarization plane relative to the direction of the dominant
orientation in the structure of the sample of biotissues of stroma of squamous cancer and cervix adenocarcinoma were
carried. Results of the investigation of the tumor tissues showed that the magnitude of the linear dichroism Δ is
insignificant in the researched spectral range λ=280-840 nm and specific regularities in its change observed short-wave
ranges.
The aim was to study the possibility of using polarimetry methods of performance evaluation of blood plasma of patients
with breast cancer and spectroscopy method in the diagnosis of breast cancer and determine the criteria for their use of
non-invasive screening for problems.
Different statistical modeling techniques of radiation propagation in epithelial tissue are considered. The two main approaches are: modified classical Monte Carlo method for light propagation in turbid medium and the coherent inverse ray tracing method. Classical Monte Carlo method was modified to take into account polarization of propagated radiation and birefringence which can occur due to tissues commonly found under epithelium. As a supplementary method modified version of classical ray tracing technique is used which takes into account phase of radiation during its propagation in tissue.
The features of formation of interferometer output in the case of a broadband light source and random medium as a retro-reflector in the interferometer object arm are considered. It is shown for the large values of the pathlength difference the interferometer output decays exponentially with the decay rate dependent on the transport parameters of a probed random medium. This allows us to determine the transport mean free path of light propagation in the medium. This approach can e applied not only for the strongly scattering media, but also for scattering samples characterized by non-diffusive regime of light propagation.
An approach to differentiation of the morphological features of normal and pathological human epidermis on the base of statistical analysis of the local polarization states of laser light forward scattered by in-vitro tissue samples is discussed. The eccentricity and the azimuth angle of local polarization ellipses retrieved for various positions of the focused laser beam on the tissue surface, and the coefficient of collimated transmittance are considered as the diagnostic parameters for differentiation. The experimental data obtained with the psoriasis, discoid lupus erythematosus, alopecia, lichen planus, scabies, demodex, and normal skin samples are presented.
Early detection of malignant transformation is a goal of modern medicine and to this end there is an impressive number of approaches from the scientific field that tries to identify early changes preceding malignant transformation in order to establish a correct diagnosis. This paper aims to combination of polarization and fluorescence techniques for identifying the changes in biotissues of growth and development of experimental solid tumors.
The basic idea of the used technique is for using stochastic approximation to simulate physical phenomena in the
propagation of polarized radiation scattering environment. In the standart modeling only absorbed, missed and reflected photons are calculated, in polarization modeling structural and geometric parameters are noticed. The flowchart contains the basic elements of the standard elements of simulation and calculation with the regard to polarization.
Early detection of malignant transformation is a goal of modern medicine and to this end there is an impressive number
of approaches from the scientific field that tries to identify early changes preceding malignant transformation in order to
establish a correct diagnosis. This paper aims to combination of polarization and fluorescence techniques for identifying
the changes in biotissues of growth and development of experimental solid tumors.
Early detection of malignant transformation is a goal of modern medicine and therefore there is an impressive number of
approaches from the scientific field that tries to identify early changes preceding malignant transformation in order to
establish a correct diagnosis. This paper aims is to combine the optical and biochemical techniques for identifying the
changes in membrane dynamics of growth and development of experimental solid tumours. The experimental Walker
256 carcinoma graft in Wistar rats has been used, followed from day 7 up to day 24 from inoculation of tumour cells.
Optical techniques were used in fractalometry laser polarization in the preclinical diagnosis of pathological changes and
degenerative-dystrophy of experimental tissue, and in terms of biochemical tumour tissue was determined the reaction of
lipid peroxidation monitored by the malondialdehyde (MDA), the end product assays of reaction. In addition the total
antioxidants as a response of the endogenous defences systems were followed, too. The results indicate a rising profile of
the processes investigated by the 14th day after tumour graft, following a decrease due to the lack of substrate enzymatic
reactions, specifically the double links of polyunsaturated fatty acids in the membranes change during tumour
development.
This work is directed to the investigation of the scope of the technique of laser polarimetry and polarization spectrometry
of oncological changes of the human prostate tissue under the conditions of multiple scattering. It was shown that the
third statistic moment in the intensity distribution proved to be the most sensitive to pathological changes in orientation
structure. Its value in the intensity distribution of polarization image I (0 - 90) of oncologically changed tissue is 21 times
higher if compared with the similar statistic parameter of the intensity distribution of the healthy tissue. The results of
studies of size linear dichroism prostate gland, as healthy and affected by malignant tumor at different stages of its
development was presented. Significant difference in the values of linear dichroism and its spectral dependence in the
spectral range λ = 280 - 840 nm as between research facilities, and between biotissues - healthy (or affected by benign
tumors) and cancer patients was shown. These results may have diagnostic value for detection and assessment of the
development of cancer.
The qualities of the polarization-correlation structure of the images of thin film of amino acid are researched. The
samples of 20 amino acid were used in the experiment on the modified micropolarimeter. Research methods are Mueller
matrices polarimetry and spectropolarimetry in 200-2000 nm, as well as spectrometry in 2500-25 000 nm. The two-dimensional
distribution of Stocks image and Mueller matrix elements of amino acid are obtained, the correlated analysis
of the necessary images was carried out. The specific for every amino acids uniaxial character of the crystals was
corroborated.
This paper follows to combine optical and biochemical techniques for identification the cell membrane transformation in
the dynamic of growth and development of experimental solid tumour. It is researched that in all the cases the linear
dichroism appears in biotissues (the human esophagus, the muscle tissue of rats, prostate tissue) with the cancer disease
the magnitude of which depends on the type of the tissue and on the time of the cancer process development. As the
linear dichroism is lacking for healthy tissues, then the obtained results can have diagnostic values with the purpose of
detection and estimation of the stage of the cancer disease development.
The qualities of the polarization-correlation structure of the images of thin film of aminoacid are researched. The
samples of 20 aminoacid were used in the experiment on the modified micropolarimeter. Research methods are Mueller
matrices polarimetry and spectropolarimetry in 200-2000 nm, as well as spectrometry in 2500-25000 nm.
The two-dimensional distribution of Stocks image and Mueller matrix elements of aminoacid are obtained, the
correlated analysis of the necessary images was carried out. The specific for every amino acids uniaxial character of the
crystals was corroborated.
The interconnection between geometry of biological tissue structure with their polarization properties has been studied. It
has been shown that for physiologically normal biological tissues polarization properties of radiation scattered on
architectonic nets formed by protein fibrils possess the fractal character. Pathological changes of biological tissues
architectonics are accompanied with the transformation of self-similar structure of Mueller-matrix images into stochastic
and statistic ones.
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