Mr. Robert N. Graf Profile

Robert N. Graf

at Duke Univ

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Publications (7)

SPIE Journal Paper | 1 November 2009 Open Access

Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations

Robert Graf, Francisco Robles, Xiaoxin Chen, Adam Wax

JBO, Vol. 14, Issue 06, 064030, (November 2009) https://doi.org/10.1117/12.10.1117/1.3269680

KEYWORDS: Tissues, Spectroscopy, Spectral resolution, Tissue optics, Optical spectroscopy, Optical coherence tomography, Light scattering, Statistical analysis, Spectral data processing, Biomedical optics

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Proceedings Article | 24 February 2009 Paper

Dual window method for processing spectroscopic optical coherence tomography signals with high spectral and spatial resolution

Francisco Robles, Robert Graf, Adam Wax

Proceedings Volume 7168, 71680W (2009) https://doi.org/10.1117/12.809465

KEYWORDS: Optical coherence tomography, Spectroscopy, Spectral resolution, Optical spectroscopy, Spatial resolution, Signal processing, Fourier transforms, Tissue optics, Time-frequency analysis, Cancer

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Proceedings Article | 24 February 2009 Paper

Detecting neoplastic development in the hamster cheek pouch using Fourier domain low coherence interferometry

Robert Graf, Francisco Robles, Xiaoxin Chen, Adam Wax

Proceedings Volume 7187, 71870T (2009) https://doi.org/10.1117/12.809494

KEYWORDS: Signal detection, Spectroscopy, Light scattering, Scattering, Tissue optics, Tissues, Imaging systems, Spectral resolution, Interferometry, Optical coherence tomography

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Proceedings Article | 11 March 2008 Paper

Measuring neoplastic transformation in the hamster cheek pouch using Fourier domain low-coherence interferometry

Robert Graf, Xiaoxin Chen, William Brown, Adam Wax

Proceedings Volume 6864, 68640P (2008) https://doi.org/10.1117/12.764045

KEYWORDS: Signal detection, Tissues, Spectroscopy, Light scattering, Natural surfaces, Interferometry, Imaging systems, Scattering, Tissue optics, Optical coherence tomography

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Fourier Domain Low Coherence Interferometry (fLCI) is a promising technique which combines the depth resolution of low coherence interferometry with the sensitivity of light scattering spectroscopy for probing the health of epithelial tissue layers. Our new fLCI system configuration utilizes a white light Xe arc lamp source and a 4-f interferometer which re-images light scattered from the sample onto the detection plane. The system employs an imaging spectrometer at the detection plane to acquire depth resolved profiles from 252 adjacent spatial points without the need for any scanning. The limited spatial coherence of the light source requires the resolution of adjacent spatial points for the generation of depth information. Depth-resolved spectral information is recovered by performing a short-time Fourier transform on the detected spectra, similar to spectroscopic optical coherence tomography. Wavelength dependent variations in scattering intensity are analyzed as a function of depth to obtain information about the neoplastic transformation of the probed cells. Previous studies have demonstrated fLCI as an excellent technique for probing the scatterer morphology of simple phantoms and of in vitro cancer cell monolayers. We now seek to assess the ability of the new fLCI system to measure the health of subsurface tissue layers using the hamster cheek pouch model. Seven hamsters will have one cheek pouch treated with the known carcinogen DMBA. At the conclusion of the 24 week treatment period the animals will be anesthetized and the cheek pouches will be extracted. We will use the fLCI optical system to measure the neoplastic transformation of the in situ subsurface tissue layers in both the normal and DMBA-treated cheek pouches. Traditional histological analysis will be used to verify the fLCI measurements. We expect our results to establish the feasibility of fLCI to distinguish between healthy and dysplastic epithelial tissues in the hamster cheek pouch.

Proceedings Article | 15 February 2007 Paper

Development of a pre-clinical Fourier domain low coherence interferometry system

Robert Graf, Adam Wax

Proceedings Volume 6446, 64460L (2007) https://doi.org/10.1117/12.699373

KEYWORDS: Light scattering, Tissues, Signal detection, Spectrographs, Imaging systems, Scattering, Interferometry, Spectroscopy, Tissue optics, Interferometers

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SPIE Journal Paper | 1 September 2005 Open Access

Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry

Adam Wax, John Pyhtila, Robert Graf, Ronald Nines, Charles Boone, Ramachandra Dasari, Michael Feld, Vernon Steele, Gary Stoner

JBO, Vol. 10, Issue 05, 051604, (September 2005) https://doi.org/10.1117/12.10.1117/1.2102767

KEYWORDS: Tissues, Tumors, Esophagus, Interferometry, Cancer, Tissue optics, Light scattering, Prototyping, Metrology, Cell death

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Proceedings Article | 13 April 2005 Paper

Nuclear morphology measurements using Fourier domain low-coherence interferometry

Robert Graf, Adam Wax

Proceedings Volume 5690, (2005) https://doi.org/10.1117/12.592227

KEYWORDS: Light scattering, Scattering, Interferometry, In vitro testing, Spectroscopy, Reflection, Microscopy, Image analysis, Coherence (optics), Data processing

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Showing 5 of 7 publications

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