Reconstructive skin surgeries drive the clinical need for non-contact objective measurements of skin elasticity. Here we demonstrate that all three of skin’s elastic constants (in-plane and out-of-plane shear moduli and an additional modulus defining skin’s tensile anisotropy) and the orientation of collagen fibers in dermis can be determined from Rayleigh wave anisotropy in-plane with acoustic micro-tapping (AuT) OCE. A nearly-incompressible transverse isotropic (NITI) model was used to reconstruct skin’s moduli from OCE measurements in human forearm in vivo for five healthy volunteers. Co-registered polarization-sensitive (PS-) OCT shows that optical and mechanical axes are co-aligned at measured sites.
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