Transport of intensity phase microscopy (TIPM) can obtain sample phase images with commercial microscope and simple procedures; however, it requires multi-focal images limiting its real-time detection capability. In order to achieve dynamics of the live cells, we adopted dual-view TIPM which simultaneously captures under- and over-focus images for phase retrieval. In dual-view TIPM, two identify CMOS cameras were first set on the binocular tube of the microscope with different focal position of 3 mm difference to simultaneously capture both the under- and over-focus images. Afterwards, scaling, rotation and shifting are all corrected to maintain the same fields of view of under- and over-focus images. Next, the cellular phase can be extracted by solving the Poisson equation. The dual-view TIPM was finally adopted in live cell imaging, proving the dual-view TIPM can obtain real-time quantitative cellular phase imaging.
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