Surface-enhanced Raman spectroscopy (SERS) has enormous potential as a highly sensitive (to a single-molecular level) and molecular-specific analytical technique for biological imaging and sensing. For the reliable SERS sensing, fabrication of metal nanostructures bundle patterns with multiple narrow nano-gaps over the homogenous macroscale is important pre-requisite due to their enhanced properties. Unfortunately, the fabrication of dense and uniform nano-gaps without organic materials is rather difficult since they usually have required complicated and a number of synthetic steps. In this research, we propose a facile and efficient methodology for manipulation of nano-gap densities inside Ag bundle patterns (ABPs) by controlling the Ag nanostructure size over a large area. Especially, we successfully demonstrate the fabrication of high-density small nano-gaps (about 2.5 nm) between silver nanostructure array patterns. We generated uniform nano-hole patterns over the entire substrate through nano-imprint lithography and silver nanostructures were deposited via electrodeposition. The relative size of Ag nanostructure elements was controlled by the Ag precursor concentration. Finally, we fully demonstrate their application in the rapid detection of rhodamine 6G (R6G) molecules by SERS with a very low detection limit as well as excellent signal uniformity, indicating an extraordinary capability for single-molecule detection.
KEYWORDS: Nanoparticles, Magnetism, Cancer, Magnetic resonance imaging, Near infrared, Tumors, Scanning electron microscopy, Oncology, Diagnostics, Transmission electron microscopy
Hybrid organic-inorganic near-infrared responsive magnetic nanoparticles were synthesized for theragnosis combined with localized therapy. In detail, inorganic super-paramagnetic nanoparticles were embedded inside organic polyaniline matrix, which enables localized photothermal therapy upon NIR illumination under intracellular acidic/oxidative condition. In this structure, super-paramagnetic nanoparticle works as MRI contrast agent, that enables the visualization of a tumor and polyaniline works for near-infrared responsive tumor ablation.
The ultrasensitive and selective diagnosis along with efficient therapy in earliest stage of cancer has been enormously highlighted by a better means to treat the cancer. Herein, Gd (III) chelated polyaniline nanoparticles (GPNPs) was synthesized to be served as a highly T1 enhanced contrast agents and Near-Infrared photothermal therapy probes. In addition, therapeutic antibody was conjugated on the surface of GPNPs for the targeted theragnosis. Fabricated GPNPs significantly reduced the T1 of water protons with as well as represented photothermal ability. The physicochemical properties and biocompatibilities of GNPSs were fully characterized and GPNPs exhibited excellent tumor targeting ability. Consequently, GPNPs exhibited strong potential for efficient diagnosis of cancer in MR imaging and photothermal ablation of epithelial cancer cells.
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