We are evaluating a range of commercially available liquid aerosol generators to produce chemical aerosols of controlled characteristics such as aerosol size, mass, and concentration that can be implemented at our Ambient Air Test Facility (AATF). For our current program of interest, we generate simulant aerosols representing classes of chemicals of interest. Various types of generators are characterized in the laboratory, including atomizing nozzles and vibrating mesh-based devices. Studies are conducted to quantify the range of aerosol size, mass, and number concertation achievable for the different types of aerosol generators. Some of the analysis and the laboratory results are presented here.
Many CBRNE threat materials are optimally implemented as aerosols. However, aerosol threats present unique challenges for sensor development, test and evaluation since particles may disburse in a turbulent atmosphere differently from tracer gases. This presentation addresses the need for tracer aerosol particles with known size distributions to be released with agent target simulant aerosols to provide ground truth for sensor test and evaluation. A novel approach for achieving uniquely identifiable individual aerosol particles is described based on utilization of quantum dots (QDs) and/or other luminescent nanocrystals (NCs), to create a multiplexed spectral barcode in tracer aerosol particles. QDs are small, typically nanometer scale, compared to micron-sized polymer beads as host aerosol particles. They also possess desirable optical properties of narrow, efficient emission bands, and are typically long-lived compared to organic dye molecules that photodegrade in sunlight. Multiple QD subpopulations, each with a narrow emission band at a distinct peak wavelength, can be encapsulated in a polymer microbead, conferring a superposition emission profile having multiple narrow peaks. The relative intensities of the emission peaks can be controlled by adjusting the number of QDs in each subpopulation. This spectral emission profile effectively becomes an individual particle barcode. Multiple polymer bead samples can be prepared each with different emission pattern (barcode). These samples can be mixed with target materials to be simultaneously released as aerosols to provide test ground truth for the simulant. Proof-of-principle experiments assessing the feasibility for using combinations of embedded NC populations in micron-sized droplets, as well as potential challenges to practical implementation will be discussed.
One of the uses of the Ambient Aerosol Test Facility (AATF) at the U.S. Naval Research Laboratory is to operate as a test facility for developmental chemical aerosol sensors. The facility draws ambient air from outside the building, with or without HEPA filtration, then introduces aerosols by various means within a 30 cm diameter, 14 m long wind tunnel at flow velocities ranging from 2 to 20 m/s. The turbulent flow generated provides a uniform distribution to a few percent across 90% of the tube diameter to sampling ports in a 3 m long test section at the end. The test section allows sampling and analysis by various sensors to determine aerosol size, concentration, and chemical composition. For the current program of interest, we generate simulant aerosols representing various classes of chemicals of interest. A range of referee instruments to characterize the aerosol in terms of size, number and composition is planned. Commercially available particle sizers and counters, a gas analyzer and an aerosol mass spectrometer are part of the suite of referee instruments. We use a high-resolution, time-of-flight aerosol mass spectrometer (HR-ToF-AMS) from Aerodyne, Inc., which is configured with an aerosol focusing lens to transmit between 50 nm and 3.5 μm diameter particles, and provides size, mass loading and chemical composition information. A calibration system consisting of a scanning mobility particle sizer and a water-based condensation particle counter is used to validate the operation of the AMS instrument. We describe the AMS instrument and its use at the AATF for the assessment of other instruments.
The use of light with orbital angular momentum (OAM) is being investigated in a wide range of fields that include material interrogation, light propagation, sensing and communication1 . The defining characteristic of the electric field of OAM light is an angular term described by an azimuthal component such as e -imφ , which produces helical phasefronts and an angular momentum equal to mħ2 . For the cases where m ≠ 0 the beams can have an intensity minima along the central axis creating a beam of light which is tube-like in structure. This, together with the angular component of the energy flux, can cause the scattering interactions with materials to be different than that from plane waves. Theoretical angular scattering calculations show that the light scattering maxima can occur at different angles from the forward direction of zero degrees. In this work we investigate the scattering properties of OAM light from single, micron sized spherical particles that are suspended in a linear electrodynamic trap. Using phase plates we generate OAM beams (wavelength of 532 nm) that are incident on a single suspended particle. Using three separate CCDs we capture the scattered light intensity over a total range across 40 degrees in the forward, back and side scattering planes. Comparisons between angular scattering measurements from Gaussian beams and OAM mode equal to 3 is presented.
We have developed a multi-spectral SWIR lidar system capable of measuring simultaneous spatial-spectral information for imaging and spectral discrimination through partial obscurations. Our system utilizes a supercontinuum laser source and eight narrowband spectral channels in the 1000 nm to 1600 nm region. The system employs a steering mirror, which enables us to scan the region of interest and collect spectral and spatial data as a point-by-point scan. The system is designed to detect weak signal returns in the few-photon regime. The technique promises more capable classification and target detection of spectrally diverse targets in obscured environments with potential applications for mapping of ground type through forest canopy, pollution monitoring of water ways, and intelligence, surveillance, reconnaissance and target detection (ISRT). Custom targets designed to provide distinct spectral response are employed to ascertain the system’s response. The lidar system is calibrated by measuring the return signal from a highly reflective flat Spectralon target; this enables us to determine the reflectivity of the objects of interest. Spectral response of the targets are analyzed and their estimated reflectivity is reported. The same targets are studied in the presence of two partial obscurants. The objects are easily identified even though the return signal is attenuated by a factor of seven. The general spectral shape of the targets are preserved in the presence of the obscurants. More challenging objects and environments and various methods to recover the spectral response of the objects are currently being pursued.
The goal of this project is to investigate correlations of polarimetric angular scattering patterns from individual aerosol particles with the particles’ physical structure and composition. Such signature patterns may be able to provide particle classification capability, such as, for example, discrimination between man-made and naturally occurring aerosols. If successful, this effort could improve current detection methods for biological warfare (BW) agent aerosols. So far, we have demonstrated an experimental arrangement to measure polarization-state resolved, multi-angle, scattering intensities from single aerosol particles on-the-fly. Our novel approach is a radical departure from conventional polarimetric measurement methods, and a key factor is the use of a multiple-order retarder to prepare different polarization states, depending on the wavelength of the incident light. This novel experimental technique uses a supercontinuum light source, an array of optical fibers, an imaging spectrometer and an EMCCD camera to simultaneously acquire wavelength and angle dependent particle light scattering data as a two-dimensional snapshot.
Mueller matrix elements were initially measured from individual particles held in an optical trap (at 405 nm). Since particles can be stably trapped for long periods (hours), we were able to change the optical configuration to acquire multiple Mueller matrix element measurements on a single particle. We have computationally modeled these measurements at specific angles, and the comparison with experimental measurements shows good agreement. Similar measurements have also been made on slowly falling particles, and our current efforts are focused on improving experimental technique sufficiently to make such measurements on flowing particles.
A dual wavelength UV-LIF fluorescence system that uses 266 nm and 355 nm laser pulses to sequentially excite
single aerosol particles has been shown to provide significant discrimination between biological and ambient as well
as differentiation among classes of biological particles. This particle classification data can then be used to trigger
an electrostatic capture mechanism to deposit individual potential bio-threats particles onto a stainless steel substrate
and particles that are not classified as targets are discharged with the exiting airflow. Timing and velocity
information for each on-the-fly particle are critical for setting an appropriate delay to capture the particles of
interest. A novel CW laser beam technique has been developed to measure the velocity of each particle and initiate a
timing sequence. The electrostatic capture mechanism then electrically charges identified particles and produces a
time-delayed electric field to drive them into the stainless steel substrate. The resulting collected sample is highly
enriched with target, or potential threat, particles in comparison to their percentage in the ambient air. This
presentation will describe the unique optical interrogation and diagnostic techniques that have been developed to
make this achievement possible, as well as provide the latest system performance results.
Sarnoff Corporation and the Naval Research Laboratory, through support of the U.S. Department of Homeland
Security, are developing an automated, high throughput bio-aerosol physical enrichment system designed for use as
part of a biological-threat protection system. The Biological Aerosol-Capture-Enrichment (BioACE) system is a
bio-aerosol collection system that combines three unique technologies to create physically enriched aerosol samples
that can be subsequently interrogated by any number of bio-threat detection systems for the presence of threat
agents. An air-to-air concentrator uses an inertial separation technique to highly concentrate an aerosol sample
presented to a dual wavelength ultra-violet laser induced fluorescence (UVLIF) optical trigger used to discriminate
potential threat particles from non-threat particles conveyed in a collimated particle stream. This particle
classification information is used to trigger an electrostatic deposition mechanism to deposit only those particles
determined to be potential bio-threats onto a stainless steel substrate. Non-threat particles are discarded with the
exiting airflow.
The goal for the most recent development effort has been the integration and optimization of these technologies into
a unit capable of producing highly enriched particulate samples from ambient air containing variable background
aerosol loading and type. Several key technical and engineering challenges were overcome during the course of this
development including a unique solution for compensating particle velocity dispersion within the airflow,
development of a real-time signal acquisition and detection algorithm for determining material type on a particle by
particle basis at rates greater than 2000 particles per second, and the introduction of a robust method for transferring
deposited particulate into a 50ul wet sample suitable for most advanced bio-detection techniques.
This paper will briefly describe the overall system architecture and then concentrate on the various component and
system design tradeoffs required to optimize sample enrichment performance. A system performance model will be
presented along with detailed analysis of the optical system components and electronic signal processing needed for
achieving high concentration sample enrichment. Experimental methods and data obtained in the laboratory setting
and from real world environments will be described and used to support the performance model of the system.
Finally, a number of air sampling scenarios will be analyzed using the system performance model to determine the
applicability of the BioACE system to the various concepts of operation perceived to be needed for achieving a high
performance bio-threat detect-to-protect system.
A bioaerosol sensor based on dual wavelength fluorescence excitation and multiple wavelength elastic
scattering has been developed and characterized for classifying micron-sized particles on the fly. The UVLIF
instrument successfully completed a field trial in which we detected and correctly identified over 90% of the simulant releases over the 2 week testing period.
Sarnoff Corporation and the Naval Research Laboratory, through support from HSARPA, are developing an automated,
high throughput bio-aerosol physical enrichment system designed for use as part of a biological-threat protection system.
The Biological Aerosol-Capture-Enrichment (BioACE) system is a bio-aerosol collection system that combines three
unique technologies to create physically enriched aerosol samples that can be subsequently interrogated by any number
of bio-threat detection systems for the presence of threat agents. An air-to-air concentrator uses an inertial separation
technique to highly concentrate an aerosol sample presented to a dual wavelength ultra-violet laser induced fluorescence
(UVLIF) optical trigger used to discriminate potential threat particles from non-threat particles conveyed in a collimated
particle stream. This particle classification information is used to trigger an electrostatic deposition mechanism to
deposit only those particles determined to be potential bio-threats onto a stainless steel substrate. Non-threat particles are
discarded with the exiting airflow. A prototype laboratory system in which particle size dependent elastic scatter rater
than fluorescence provides the triggering signal has been experimentally qualified. This paper will present a detailed
overview of the prototype system and discuss the physical enrichment results achieved.
A partnership that includes the Naval Research Laboratory (NRL), MIT Lincoln Laboratories and the Edgewood Chemical and Biological Command is engaged in an effort to develop optical techniques for the rapid detection and classification of biological aerosols. This paper will describe two efforts at NRL: development of an improved UV fluorescence front-end trigger and the use of infrared absorption spectroscopy to classify biological aerosol particles. UV Laser-induced fluorescence (UVLIF) has been demonstrated to provide very high sensitivity for differentiating between biological and inorganic aerosol particles. Unfortunately, current UVLIF systems have unacceptably high false alarm rates due to interferences from man made and naturally occurring organic and biological particulates. We have developed a two-wavelength, UVLIF technique that offers a higher level of discrimination than is possible using single wavelength UVLIF. Infrared absorption spectroscopy coupled with multivariate analysis demonstrates a high potential for differentiation among members of biological and chemical sample classes. Two-wavelength UVLIF in combination with the IR interrogation of collected bioaerosols could provide a rapid, reagentless approach to specific classification of biological particles according to an operational level of discrimination - the degree of particle characterization required in order to signal the presence of pathogenic material.
A two-wavelength excitation bioaerosol sensor has been developed and characterized for classifying various types of aerosols, including biological organisms and non-biological interferents. Single aerosols, smaller than 10 μm, are interrogated with 266 nm and 355 nm laser pulses separated in time by 400 ns. Fluorescence signals excited by these pulses are detected in three broad spectral bands centered at 350 nm, 450 nm and 550 nm. The results indicate that bacterial spores, vegetative bacterial cells and proteins can be differentiated based on the two wavelength excitation approach.
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