Multiplex Coherent Anti-Stokes Raman Scattering (M-CARS) is an innovative nonlinear spectroscopic approach designed to characterize the vibrational modes of molecular structures. Coherent Raman scattering has been used for the characterization of biomedical targets for about 20 years and the multiplex aspect was introduced 10 years ago thanks to the use of a supercontinuum laser system. For each of these systems, the combination of a pump and a probe wave, driven by an external delay line, is however required to produce the vibrations. In the present work, we propose a new M-CARS system, free of the external delay line. A few-mode microstructured fiber enables merging both wave-packets (pump and supercontinuum) within a single waveguide. We showcase the capability of this system in generating hyperspectral images of biochemically active compounds. Curcumin I, the principal yellow compound isolated from Curcuma longa (Turmeric), is distinguishable by its multiple functional groups that display a nonlinear spectroscopic behavior.
Photodynamic therapy (PDT) using porphyrins has been approved in treatment of several solid tumors thanks to generation of cytotoxic reactive oxygen species (ROS). However, low physiological solubility and lack of selectivity towards tumors sites are the main limitations of their clinical use. Nanoparticles are able to spontaneously accumulate in solid tumors through the enhanced permeability and retention (EPR) effect due to leaky vasculature, poor lymphatic drainage and increased vessel permeability. Herein, we demonstrated added value of nanoparticles vectorization strategy on anticancer efficacy of the 5-(4-hydroxyphenyl)-10,15,20-triphenylporphyrin (TPPOH). Using the 80 nm silica nanoparticles (SNPs) coated with xylan-TPPOH conjugate (TPPOH-X), we highlighted on HT-29 tumor-bearing nude mice, a high anticancer efficacy of TPPOH-X SNPs compared to TPPOH free.
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