We present a method of high resolution, non-invasive, in vivo vascular imaging obtained using watersoluble and bright SWIR-emitting gold nanoclusters presenting an anisotropic surface charge combined with SWIR detection and Monte Carlo processing of the images. We applied this approach to quantify vessel complexity in mice presenting vascular disorders.
Theranostics is an innovative research field that aims to develop high target specificity cancer treatments by administering small metal-based nanoparticles (NPs). This new generation of compounds exhibits diagnostic and therapeutic properties due to the high atomic number of their metal component. In the framework of a combined research program on low dose X-ray imaging and theranostic NPs, X-ray Phase Contrast Tomography (XPCT) was performed at ESRF using a 3 μm pixel optical system on two samples: a mouse brain bearing melanoma metastases injected with gadolinium NPs and, a mouse liver injected with gold NPs. XPCT is a non-destructive technique suitable to achieve the 3D reconstruction of a specimen and, widely used at micro-scale to detect abnormalities of the vessels, which are associated to the tumor growth or to the development of neurodegenerative diseases. Moreover, XPCT represents a promising and complementary tool to study the biodistribution of theranostic NPs in biological materials, thanks to the strong contrast with respect to soft tissues that metal-based NPs provide in radiological images. This work is relied on an original imaging approach based on the evaluation of the contrast differences between the images acquired below and above K-edge energies, as a proof of the certain localization of NPs. We will present different methods aiming to enhance the localization of NPs and a 3D map of their distribution in large volume of tissues.
Fluorescent chemical sensors have been widely exploited for the detection and quantification of trace explosives. Their
performances are mainly dependent on the sensitive material. Fluorescent organic materials are very efficient in terms of
sensitivity, selectivity and response time but their degradation is a major drawback. Sol-gel materials offer a much longer
life span, especially in the case of inorganic sensitive coatings. The elaboration of sol-gel sensitive films is detailed in
this paper. Two examples of sol-gel fluorescent sensitive materials are presented: a hybrid organic-inorganic film and an
inorganic material.
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