Mr. Xingjian Zhong Profile

Xingjian Zhong

at Boston Univ Photonics Ctr

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Author

Publications (5)

Proceedings Article | 13 March 2024 Presentation + Paper

Quantitative multiplexed SWIR imaging to study nanomedicine surface coatings in vivo

Xingjian Zhong, Amish Patel, Yidan Sun, Mallory Moffett, Allison Dennis

Proceedings Volume 12859, 1285902 (2024) https://doi.org/10.1117/12.3003317

KEYWORDS: Colloidal quantum dots, Nanoparticles, Biological imaging, Short wave infrared radiation, Multiplexing, Lymphatic system, Fluorescence imaging, In vivo imaging

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SPIE Journal Paper | 5 April 2023 Open Access

Minimizing near-infrared autofluorescence in preclinical imaging with diet and wavelength selection

Yidan Sun, Xingjian Zhong, Allison M. Dennis

JBO, Vol. 28, Issue 09, 094805, (April 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.9.094805

KEYWORDS: Autofluorescence, Emission wavelengths, Fluorescence imaging, Tunable filters, Biological imaging, Imaging systems, Image filtering, Tissues, Preclinical imaging, Light sources and illumination

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SignificancePreclinical fluorescence imaging with NIR-I (700 to 900 nm) illumination and short-wave infrared or NIR-II (1000 to 1700 nm) emission increases tissue penetration depth and improves resolution through decreased scattering. Background autofluorescence decreases signal-to-background ratios (SBR) in fluorescence imaging; maximizing SBR will further improve the impact of deep tissue imaging.AimThe impact of rodent diet, illumination wavelength, and emission range on the background fluorescence and contrast agent SBR were determined to assist with the experimental design of future imaging studies.ApproachFollowing illumination with 670, 760, or 808 nm, autofluorescence in the NIR-I (<975 nm), NIR-II (>1000 nm), and NIR-II LP (>1250 nm) regions was assessed in mice fed chow or a purified diet using an IR VIVO preclinical imager (Photon, Etc.). Comparison of the SBR of liver-localized indocyanine green in the various imaging conditions indicated when gut autofluorescence was a problematic confounder.ResultsMice fed chow exhibit high levels of background autofluorescence in the gastrointestinal tract and, to a lesser extent, skin when illuminated with 670 nm light for NIR-I imaging (700 to 975 nm), interfering with the identification of fluorescently labeled tissue. Background autofluorescence was reduced by more than two orders of magnitude by any of the following changes: (1) purified diet; (2) excitation with 760 or 808 nm illumination; or (3) emission in the NIR-II (1000 to 1600 or 1250 to 1600 nm). Although the SBR was generally sufficient for feature identification except when imaging of chow-fed mice with 670 nm excitation and NIR-I emission, switching to a purified diet, using longer excitation wavelengths, or using longer emission wavelengths improved SBR significantly.ConclusionsSystematic comparison of imaging conditions and diet highlights the reduction in autofluorescence and increase in SBR enabled by intentional choices in the experimental parameters including diet, excitation wavelength, and emission wavelength range.

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Proceedings Article | 17 March 2023 Presentation

PbS/CdS QDs enabled SWIR imaging for lymphatic mapping and multiplexed lymph node imaging (Conference Presentation)

Xingjian Zhong, Amish Patel, Allison Dennis

Proceedings Volume PC12395, PC123950A (2023) https://doi.org/10.1117/12.2649410

KEYWORDS: Lymphatic system, Short wave infrared radiation, Multiplexing, Tissues, Lead, Cadmium sulfide, Visibility, Signal to noise ratio, Semiconductors, Scattering

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Proceedings Article | 9 March 2023 Poster + Paper

Biodegradable and biocompatible semiconductor nanocrystals as NIR-II photoacoustic imaging contrast agents

Vinoin Devpaul Vincely, Swathi Katakam, Kristie Huda, Xingjian Zhong, Joshua Kays, Allison Dennis, Carolyn Bayer

Proceedings Volume 12379, 1237915 (2023) https://doi.org/10.1117/12.2646501

KEYWORDS: Contrast agents, Signal generators, Animals, Semiconductors, Particles, Nanoparticles, Nanocrystals, Image restoration, Photoacoustic imaging, Near infrared

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Proceedings Article | 3 March 2022 Presentation

Engineering PbS/CdS quantum dots for NIR-II multiplexed imaging

Xingjian Zhong, Alexander Saeboe, Umar Iqbal, Dongling Zhang, Maria Moreno, Allison Dennis

Proceedings Volume PC11977, PC1197702 (2022) https://doi.org/10.1117/12.2607967

KEYWORDS: Quantum dots, Multiplexing, Atmospheric particles, Short wave infrared radiation, Quantum efficiency, Infrared radiation, Infrared imaging, In vivo imaging, Signal to noise ratio, Shortwaves

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