Progress achieved in the field of stem-cell technology allows the reprogramming of patient-derived cells, obtained from urine or skin biopsies, into induced pluripotent stem cells that can then be differentiated into any cell types. Within this framework, techniques, being able to accurately and non-invasively characterize cell structure, morphology, and dynamics, represent very promising approaches to identify disease-specific cell phenotypes. Consequently, we will present how a label-free optofluidic platform, based on quantitative-phase digital holographic microscopy along with various experimental developments in microfluidics, constitutes a very appealing cell imaging methodology to identify, through the measurement of biophysical properties, specific cell phenotypes.
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