The digital holography technology is based on holography. A photodetector device is used instead of a holographic dry plate as a recording medium. The light intensity and phase information of the object is recorded, and the reproduced image is obtained by using a computer to realize the holographic reproduction process. Digital holography can perform noncontact, high-quality measurements and can obtain three-dimensional position information. This paper presents an off-axis digital holographic underwater microbiological detection method based on Jamin interferometer. For the identification of underwater microorganisms, the Euclidean distance identification method based on Hu invariant moments was used to identify underwater microorganisms. The experimental results show that the off-axis digital holography system constructed in this paper can clearly capture the underwater microorganisms, and effectively identify the paramecium and the feasibility of the verification system and algorithm.
The course of “Applied Optics” is professional and foundational for the specialty of photo-electric information and engineering. According to the characteristics of the specialty, the teaching contents, teaching means, innovations and appraisal methods are mainly discussed in this paper. Firstly, one of the most difficult part to comprehend, the Fermat principle is taken as an example in the teaching content. By using the development history of optics and interesting natural phenomenon, students' understanding of the optical knowledge can be enhanced. Secondly, in various means of teaching art, ZEMAX provides students with a platform of training innovative consciousness and engineering capacity, and it make high cohesion in teaching and scientific research. Thirdly, in the teaching innovation, photoelectric contest can stimulate students' innovative thinking, innovation awareness, and cultivate undergraduate students’ optics, mechanics, electricity, numerology integrated design capabilities. Lastly, the reform in the appraisal methods guide students from focusing on the examination results to pay attention to the learning process. Eventually, students' study interest has improved, demand of the engineering practice has adapted, and the well teaching effect has realized.
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