The optical properties of small and highly absorbing tissues can be determined by measurement of spatially resolved diffuse reflectance at short source-detector separations. Spatial resolution and number of measuring point influence the inverting precision of optical property directly from the experimental diffuse reflectance. To increase spatial resolution and number of measuring point, a high-resolution and multiple points detection system is designed. A special optical fiber array probe is employed. Its spatial resolution is 0.125mm. The system is proved to be reliable by comparing the experimental result of diffuse reflectance from small region 0.125mm-1.25mm with that of numerical simulation. The inverting method based on Monte Carlo simulation is designed, by which optical properties can be achieved by building optical parameter date base and training artificial neural network (ANN).
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