Special Section: Selected Papers from the 4th Mediterranean Conference on Nanophotonics

Nanofabrication and plasma polymerization assisted surface modification of a transducer based on localized surface plasmon resonance of gold nanostructure arrays for biosensor applications

[+] Author Affiliations
Adil Burak Turhan

Bilkent University Nanotechnology Research Center—NANOTAM, Ankara 06800, Turkey

Hacettepe University, Institute for Pure and Applied Sciences, Plasma Aided Bioengineering and Biotechnology (PABB) Research Group, Nanomedicine and Nanotechnology Division, Ankara 06532, Turkey

Demet Ataman, Mehmet Mutlu

Hacettepe University, Institute for Pure and Applied Sciences, Plasma Aided Bioengineering and Biotechnology (PABB) Research Group, Nanomedicine and Nanotechnology Division, Ankara 06532, Turkey

Yasin Sen

Hacettepe University, Institute for Pure and Applied Sciences, Plasma Aided Bioengineering and Biotechnology (PABB) Research Group, Nanomedicine and Nanotechnology Division, Ankara 06532, Turkey

Aksaray University, Department of Food Technology, Guzelyurt Vocational School, Aksaray 06100, Turkey

Ekmel Özbay

Bilkent University Nanotechnology Research Center—NANOTAM, Ankara 06800, Turkey

Bilkent University, Department of Physics, Ankara 06800, Turkey

Bilkent University, Department of Electrical and Electronics Engineering, Ankara 06800, Turkey

J. Nanophoton. 6(1), 061602 (Jul 31, 2012). doi:10.1117/1.JNP.6.061602
History: Received April 17, 2012; Revised May 31, 2012; Accepted June 5, 2012
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Abstract.  The nanofabrication and surface modification of a transducer based on localized surface plasmon resonance (LSPR) of gold nanostructure arrays for biosensing was studied. We used electron beam lithography for the nanopatterning technique, which let us choose LSPR sensor properties by providing immense control over nanostructural geometry. A critical step in the utilization of this transducer is to form a selective biolayer over the gold nanostructures. We applied plasma polymerization and wet chemistry techniques for ethylenediamine (EDA) modification and glutaraldehyde immobilization as intermediate layers, respectively. The gold nanostructure arrays were primarily modified using EDA in order to activate the surface with amine groups that are cross-linked with later added avidin molecules by the help of glutaraldhyde layer residing in between. The success of plasma polymerization was validated with x-ray photoelectron spectroscopy measurements. As a last step, we introduced biotin to the surface (biotin has a high affinity for avidin). We were able to detect the LSPR resonance wavelength shift in the transmission spectra at each step of modification, including the avidin-biotin interaction, which acts as a model for specific molecule detection using LSPR.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Adil Burak Turhan ; Demet Ataman ; Yasin Sen ; Mehmet Mutlu and Ekmel Özbay
"Nanofabrication and plasma polymerization assisted surface modification of a transducer based on localized surface plasmon resonance of gold nanostructure arrays for biosensor applications", J. Nanophoton. 6(1), 061602 (Jul 31, 2012). ; http://dx.doi.org/10.1117/1.JNP.6.061602


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