Numerical modeling of scanning near-field optical microscopy for fluorescence-less DNA detection

Chanachai Poosri; Atikrit Chanjavanakul; Waleed S. Mohammed

doi:10.1117/12.863533

19 May 2010 Numerical modeling of scanning near-field optical microscopy for fluorescence-less DNA detection

Chanachai Poosri, Atikrit Chanjavanakul, Waleed S. Mohammed

Proceedings Volume 7743, Southeast Asian International Advances in Micro/Nanotechnology; 77430I (2010) https://doi.org/10.1117/12.863533
Event: Southeast Asian International Advances in Micro/Nano-technology, 2010, Bangkok, Thailand

Abstract

Scanning near-field optical microscopy (SNOM) has gained wide interest as a viable microscopic technique for the study of surface properties at the nanoscale. SNOM uses optical fiber to detect evanescent wave which provides a high resolution imaging beyond the diffraction limit. The collected intensity is influenced by complex refractive index of the sample. This project exploits the property of evanescent wave to discriminate between unhybridized and hybridized DNA which has a significant difference in complex refractive index. The concept could become a promising alternative since it circumvents fluorescence-labeling problems. The research focuses on numerical modeling by mean of finitedifference beam propagation and DNA hybridization based on empirical data from literatures.

Citation Download Citation

Chanachai Poosri, Atikrit Chanjavanakul, and Waleed S. Mohammed "Numerical modeling of scanning near-field optical microscopy for fluorescence-less DNA detection", Proc. SPIE 7743, Southeast Asian International Advances in Micro/Nanotechnology, 77430I (19 May 2010); https://doi.org/10.1117/12.863533

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Near field scanning optical microscopy

Refractive index

Optical microscopy

Beam propagation method

Numerical modeling

Glasses

Reflection

Show All Keywords

Keywords/Phrases

Search In:

Publication Years