Progress on multimodal retinal Imaging via OCT multi-volume averaging and two photon excited fluorescence

William Newberry; Laura Vargas; Marinko V. Sarunic

doi:10.1117/12.2609913

7 March 2022 Progress on multimodal retinal Imaging via OCT multi-volume averaging and two photon excited fluorescence

William Newberry, Laura Vargas, Marinko V. Sarunic

Proceedings Volume PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI; PC119480G (2022) https://doi.org/10.1117/12.2609913
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present our progress on multi-modal imaging in the mouse retina using OCT and Two-Photon Excited Fluorescence (TPEF). In order to progress this modality towards a clinical setting, the power incident on the retina must be reduced. With a significantly dimmer TPEF signal, motion corrected registration and averaging becomes difficult. We have developed an approach to utilize multi-modal simultaneous acquisition to non-rigidly register both datasets solely using the OCT signal. Image quality is further enhanced by correcting wavefront aberrations introduced from the high NA configuration through a sensorless image-based hill-climbing algorithm.

Conference Presentation

Citation Download Citation

William Newberry, Laura Vargas, and Marinko V. Sarunic "Progress on multimodal retinal Imaging via OCT multi-volume averaging and two photon excited fluorescence", Proc. SPIE PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, PC119480G (7 March 2022); https://doi.org/10.1117/12.2609913

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