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16 March 2023 Broadband CARS microscopy in the entire fingerprint region for biological applications (Conference Presentation)
Federico Vernuccio, Arianna Bresci, Alejandro De la Cadena, Chiara Ceconello, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, Dario Polli
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Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC123910B (2023) https://doi.org/10.1117/12.2650231
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We present a novel broadband coherent anti-Stokes Raman scattering (B-CARS) approach, employing a femtosecond fiber laser at 1035 nm and 2 MHz repetition rate. These characteristics of the driving laser enable to generate broadband Stokes pulses through white-light supercontinuum in bulk media and to use higher pulse energy to shine the sample, thus producing stronger signals. In this way, we demonstrate state-of-the-art acquisition speed (<1 ms/pixel) with unprecedented sensitivity (≈14.1 mmol/L), covering the whole fingerprint vibrational region. The B-CARS microscope, coupled with a deep learning-based post-processing pipeline, delivers high-quality hyperspectral images identifying the main Raman features of heterogeneous biological tissues.
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Federico Vernuccio, Arianna Bresci, Alejandro De la Cadena, Chiara Ceconello, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, and Dario Polli "Broadband CARS microscopy in the entire fingerprint region for biological applications (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC123910B (16 March 2023); https://doi.org/10.1117/12.2650231
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KEYWORDS
Microscopy
Raman spectroscopy
Hyperspectral imaging
Laser crystals
Microscopes
Picosecond phenomena
Signal generators
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