Investigation Of DNA's By A New Pseudo-Raman Technique

J. P. Biscar; N. Kollias; P . D. Bear

doi:10.1117/12.953901

1 May 1974 Investigation Of DNA's By A New Pseudo-Raman Technique

J. P. Biscar, N. Kollias, P . D. Bear

Proceedings Volume 0043, Application of Optical Instrumentation in Medicine II; (1974) https://doi.org/10.1117/12.953901
Event: Application of optical Instrumentation in Medicine II, 1973, Chicago, United States

Abstract

The Raman spectrum of long chain biological polymers displays a broad band which is distinct from conventional molecular Raman lines. A hypothesis based on a new Electromagnetic Molecular Electronic Resonance has been advanced to explain it. The resolved broad band data of Poly-L-Glutamic acid and Bovine Serum Albumin coincide with the predictions of this theory. The technique has been extended to the analysis of double stranded DNA's. . Spectra obtained from T7 and A viruses show that there is an Electromagnetic Molecular Electronic Resonance taking place along 1 orbital chains between coupled bases. This provides the first experimental proof that there is a IT orbital coupling from base to base in double stranded DNA's. A base dislocation along the DNA is detected in the spectrum. An important feature of this new technique is that it does identify a virus DNA, and measures its length, when the latter is still inside its protein coat.

Citation Download Citation

J. P. Biscar, N. Kollias, and P . D. Bear "Investigation Of DNA's By A New Pseudo-Raman Technique", Proc. SPIE 0043, Application of Optical Instrumentation in Medicine II, (1 May 1974); https://doi.org/10.1117/12.953901

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