Detection of mast cell secretion by using surface enhanced Raman scattering

Juan Li; Ren Li; Liqin Zheng; Yuhua Wang; Shusen Xie; Juqiang Lin

doi:10.1117/12.2245580

31 October 2016 Detection of mast cell secretion by using surface enhanced Raman scattering

Juan Li, Ren Li, Liqin Zheng, Yuhua Wang, Shusen Xie, Juqiang Lin

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 1002429 (2016) https://doi.org/10.1117/12.2245580
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Acupuncture can cause a remarkable increase in degranulation of the mast cells, which has attracted the interest of researchers since the 1980s. Surface-enhanced Raman scattering (SERS) could obtain biochemical information with high sensitivity and specificity. In this study, SERS was used to detect the degree of degranulation of mast cells according to different incubate time. Mast cells was incubated with culture medium for 0 h, 12 h and 24 h, then centrifuge the culture medium, decant the supernatant, and discard the mast cell. SERS was performed to obtain the biochemical fingerprinting signatures of the centrifuged medium. The spectra data are then analyzed by spectral peaks attribution and the principal component analysis (PCA). The measured Raman spectra of the two groups were separated well by PCA. It indicated that mast cells had secreted some substances into cultured medium though degranulation did not happen.

Citation Download Citation

Juan Li, Ren Li, Liqin Zheng, Yuhua Wang, Shusen Xie, and Juqiang Lin "Detection of mast cell secretion by using surface enhanced Raman scattering", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002429 (31 October 2016); https://doi.org/10.1117/12.2245580

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KEYWORDS

Principal component analysis

Surface enhanced Raman spectroscopy

Raman scattering

Raman spectroscopy

Proteins

Solids

Lithium

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