Amphiphilic block copolymer-based photonic platform towards efficient protein detection

Afroditi Petropoulou; Thomas J. Gibson; Efrosyni Themistou; Stergios Pispas; Christos Riziotis

doi:10.1117/12.2246506

14 November 2016 Amphiphilic block copolymer-based photonic platform towards efficient protein detection

Afroditi Petropoulou, Thomas J. Gibson, Efrosyni Themistou, Stergios Pispas, Christos Riziotis

Proceedings Volume 10025, Advanced Sensor Systems and Applications VII; 100250M (2016) https://doi.org/10.1117/12.2246506
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

The development of a low complexity fiber optic based protein sensor by functionalizing the surface of silica optical fibers using block copolymers having both hydrophobic poly(methyl methacrylate) (PMMA) and hydrophilic poly[2- (dimethylamino)ethyl methacrylate] (PDMAEMA) blocks is presented here. The amphiphilic thiol-functionalized PMMA₁₁₇-b-P(DMAEMA₁₇-st-TEMA₂) and vinyl-sulfone PMMA₁₁₇-b-P(DMAEMA₁₇-st-VSTEMA₂) block copolymers designed and synthesized in this work contain a cationic hydrophilic PDMAEMA block that can electrostatically bind selected oppositely charged proteins and also appropriate functional groups for reversible or non-reversible protein binding, respectively, leading to a refractive index change of the overlayer and hence, enabling the sensing. The developed PMMA₁₁₇-b-PDMAEMA₁₆-based platform has been evaluated for bovine serum albumin (BSA) sensing, exhibiting linear response to detected BSA concentrations.

Citation Download Citation

Afroditi Petropoulou, Thomas J. Gibson, Efrosyni Themistou, Stergios Pispas, and Christos Riziotis "Amphiphilic block copolymer-based photonic platform towards efficient protein detection", Proc. SPIE 10025, Advanced Sensor Systems and Applications VII, 100250M (14 November 2016); https://doi.org/10.1117/12.2246506

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