High-throughput optofluidic profiling of Euglena gracilis with morphological and chemical specificity

Baoshan Guo; Cheng Lei; Takuro Ito; Yiyue Jiang; Yasuyuki Ozeki; Keisuke Goda

doi:10.1117/12.2245836

4 November 2016 High-throughput optofluidic profiling of Euglena gracilis with morphological and chemical specificity

Baoshan Guo, Cheng Lei, Takuro Ito, Yiyue Jiang, Yasuyuki Ozeki, Keisuke Goda

Proceedings Volume 10026, Real-time Photonic Measurements, Data Management, and Processing II; 100260L (2016) https://doi.org/10.1117/12.2245836
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

The world is faced with environmental problems and the energy crisis due to the combustion and depletion of fossil fuels. The development of reliable, sustainable, and economical sources of alternative fuels is an important, but challenging goal for the world. As an alternative to liquid fossil fuels, algal biofuel is expected to play a key role in alleviating global warming since algae absorb atmospheric CO2 via photosynthesis. Among various algae for fuel production, Euglena gracilis is an attractive microalgal species as it is known to produce wax ester (good for biodiesel and aviation fuel) within lipid droplets. To date, while there exist many techniques for inducing microalgal cells to produce and accumulate lipid with high efficiency, few analytical methods are available for characterizing a population of such lipid-accumulated microalgae including E. gracilis with high throughout, high accuracy, and single-cell resolution simultaneously. Here we demonstrate a high-throughput optofluidic Euglena gracilis profiler which consists of an optical time-stretch microscope and a fluorescence analyzer on top of an inertial-focusing microfluidic device that can detect fluorescence from lipid droplets in their cell body and provide images of E. gracilis cells simultaneously at a high throughput of 10,000 cells/s. With the multi-dimensional information acquired by the system, we classify nitrogen-sufficient (ordinary) and nitrogen-deficient (lipid-accumulated) E. gracilis cells with a low false positive rate of 1.0%. This method provides a promise for evaluating the efficiency of lipid-inducing techniques for biofuel production, which is also applicable for identifying biomedical samples such as blood cells and cancer cells.

Citation Download Citation

Baoshan Guo, Cheng Lei, Takuro Ito, Yiyue Jiang, Yasuyuki Ozeki, and Keisuke Goda "High-throughput optofluidic profiling of Euglena gracilis with morphological and chemical specificity", Proc. SPIE 10026, Real-time Photonic Measurements, Data Management, and Processing II, 100260L (4 November 2016); https://doi.org/10.1117/12.2245836

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Signal detection

Biofuels

Flow cytometry

Microfluidics

Imaging systems

Beam splitters

Show All Keywords

Keywords/Phrases

Search In:

Publication Years