Light induced increases of photoreceptor layer reflectance in response to rhodopsin bleaching in mice measured in vivo with optical coherence tomography (Conference Presentation)

Pengfei Zhang; Mayank Goswami; Edward N. Pugh Jr.; Robert J. Zawadzki

doi:10.1117/12.2251811

16 May 2017 Light induced increases of photoreceptor layer reflectance in response to rhodopsin bleaching in mice measured in vivo with optical coherence tomography (Conference Presentation)

Pengfei Zhang, Mayank Goswami, Edward N. Pugh Jr., Robert J. Zawadzki

Author Affiliations +

Proceedings Volume 10045, Ophthalmic Technologies XXVII; 100450F (2017) https://doi.org/10.1117/12.2251811
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We have recently reported observations of light-induced broadband fundus reflectance changes in two most commonly used strains of laboratory mice, C57Bl/6J (pigmented) and Balb/c (un- pigmented albino). The action spectrum of the reflectance increase corresponded to the absorption spectrum of mouse rhodopsin in situ. Spectral changes in mouse fundus reflectivity were calculated from measurements made by broadband spectrometer, interfaced with our mouse retinal SLO system, obtained before and after bleaching. This results were fitted with a model of mouse fundus reflectance, quantifying contributions from loss of rhodopsin absorption with bleaching, absorption by oxygenated hemoglobin (HbO2) in the choroid (Balb/c), and absorption by melanin (C57Bl/6J) additionally both mouse strains exhibited light-induced broadband reflectance changes explained as bleaching-induced reflectivity increases at photoreceptor inner segment/outer segment (IS/OS) junctions and OS tips. Here we present results investigating the kinetics of the increases in reflectivity with Optical Coherence Tomography operating in a 780-950 nm band.

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Pengfei Zhang, Mayank Goswami, Edward N. Pugh Jr., and Robert J. Zawadzki "Light induced increases of photoreceptor layer reflectance in response to rhodopsin bleaching in mice measured in vivo with optical coherence tomography (Conference Presentation)", Proc. SPIE 10045, Ophthalmic Technologies XXVII, 100450F (16 May 2017); https://doi.org/10.1117/12.2251811

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KEYWORDS

Reflectivity

Absorption

Optical coherence tomography

In vivo imaging

Scanning laser ophthalmoscopy

Spectroscopy

Current controlled current source

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