Quantitative phase imaging of platelet: assessment of cell morphology and function

Irina Vasilenko; Elizaveta Vlasova; Vladislav Metelin; B. Agadzhanjan; R. Lyfenko

doi:10.1117/12.2253160

16 February 2017 Quantitative phase imaging of platelet: assessment of cell morphology and function

Irina Vasilenko, Elizaveta Vlasova, Vladislav Metelin, B. Agadzhanjan, R. Lyfenko

Proceedings Volume 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV; 100681X (2017) https://doi.org/10.1117/12.2253160
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

It is well known that platelets play a central role in hemostasis and thrombosis, they also mediate tumor cell growth, dissemination and angiogenesis. The purpose of the present experiment was to evaluate living platelet size, function and morphology simultaneously in unactivated and activated states using Phase-Interference Microscope “Cytoscan” (Moscow, Russia). We enrolled 30 healthy volunteers, who had no past history of aeteriosclerosis-related disorders, such as coronary heart disease, cerebrovascular disease, hypertention, diabetes or hyperlipidemia and 30 patients with oropharynx cancer. We observed the optic-geometrical parameters of each isolated living cell and the distribution of platelets by sizes have been analysed to detect the dynamics of cell population heterogeneity. Simultaneously we identified 4 platelet forms that have different morphological features and different parameters of size distribution. We noticed that morphological platelet types correlate with morphometric platelet parameters. The data of polymorphisms of platelet reactivity in tumor progression can be used to improve patient outcomes in the cancer prevention and treatment. Moreover morphometric and functional platelet parameters can serve criteria of the efficiency of the radio- and chemotherapy carried out. In conclusion the computer phase-interference microscope provides rapid and effective analysis of living platelet morphology and function at the same time. The use of the computer phase-interference microscope could be an easy and fast method to check the state of platelets in patients with changed platelet activation and to follow a possible pharmacological therapy to reduce this phenomenon.

Citation Download Citation

Irina Vasilenko, Elizaveta Vlasova, Vladislav Metelin, B. Agadzhanjan, and R. Lyfenko "Quantitative phase imaging of platelet: assessment of cell morphology and function", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100681X (16 February 2017); https://doi.org/10.1117/12.2253160

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KEYWORDS

Cancer

Tumors

Blood

Phase imaging

Oncology

Refractive index

Statistical analysis

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