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24 April 2017 Widely tunable, high peak power ultrafast laser sources in biological imaging (Conference Presentation)
Julien Klein
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Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100690K (2017) https://doi.org/10.1117/12.2253052
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
Widely tunable ultrafast lasers have enabled a large number of biological imaging techniques including point scanning multiphoton excited fluorescence (MPEF), SHG/THG and stimulated Raman imaging. Tunable ultrafast lasers offer spectral agility, covering the entire relative transparency window in live tissue (700-1300nnm) and flexibility with multi-color, synchronized outputs to support sophisticated label free techniques (e.g. stimulated Raman modalities). More recently newly available high peak power lasers based on Ytterbium technology drive advances in two-photon light-sheet, 3 photon excited fluorescence and holographic patterning for optogenetics photo-stimulation. These laser platforms offer a unique blend of compactness, ease of use and cost efficiency, and ideally complement tunable platforms typically based on Ti:Sapphire and IR optical parametric oscillators (OPO). We present various types of ultrafast laser architectures, link their optical characteristics to key bio-imaging requirements, and present relevant examples and images illustrating their impact in biological science. In particular we review the use of ultrafast lasers in optogenetics and fast in-vivo Calcium imaging deep in mouse brain.
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Julien Klein "Widely tunable, high peak power ultrafast laser sources in biological imaging (Conference Presentation)", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690K (24 April 2017); https://doi.org/10.1117/12.2253052
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KEYWORDS
Ultrafast lasers
Luminescence
Optical parametric oscillators
Optogenetics
Raman spectroscopy
Biomedical optics
Laser applications
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