Focus-tunable lens in limited-angle holographic tomography

A. Kuś; W. Krauze; M. Kujawińska

doi:10.1117/12.2251462

17 February 2017 Focus-tunable lens in limited-angle holographic tomography

A. Kuś, W. Krauze, M. Kujawińska

Proceedings Volume 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV; 1007009 (2017) https://doi.org/10.1117/12.2251462
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

In this paper a new, hardware-based solution for extending the depth of field in holographic tomography is presented. The solution is based on a 4f system and an electric, focus-tunable lens, which provides fast, motion-free defocusing of the plane conjugate with the camera, which acquires holograms. The optimum parameters for the required axial scanning are provided for a specific model of a commercially available tunable lens. Then, the quality of the system equipped with the designed module is analyzed and the reconstruction of a standard object (microsphere) scanned by the 4f-based defocusing system is presented. Finally, the result of the increased depth of field in the measurement domain is demonstrated with a reconstruction of a mouse fibroblast cell.

Citation Download Citation

A. Kuś, W. Krauze, and M. Kujawińska "Focus-tunable lens in limited-angle holographic tomography", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 1007009 (17 February 2017); https://doi.org/10.1117/12.2251462

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