3D/4D multiscale imaging in acute lymphoblastic leukemia cells: visualizing dynamics of cell death

Sreelatha Sarangapani; Rosmin Elsa Mohan; Ajeetkumar Patil; Matthew J. Lang; Anand Asundi

doi:10.1117/12.2270979

13 June 2017 3D/4D multiscale imaging in acute lymphoblastic leukemia cells: visualizing dynamics of cell death

Sreelatha Sarangapani, Rosmin Elsa Mohan, Ajeetkumar Patil, Matthew J. Lang, Anand Asundi

Proceedings Volume 10449, Fifth International Conference on Optical and Photonics Engineering; 1044936 (2017) https://doi.org/10.1117/12.2270979
Event: Fifth International Conference on Optical and Photonics Engineering, 2017, Singapore, Singapore

Abstract

Quantitative phase detection is a new methodology that provides quantitative information on cellular morphology to monitor the cell status, drug response and toxicity. In this paper the morphological changes in acute leukemia cells treated with chitosan were detected using d’Bioimager a robust imaging system. Quantitative phase image of the cells was obtained with numerical analysis. Results show that the average area and optical volume of the chitosan treated cells is significantly reduced when compared with the control cells, which reveals the effect of chitosan on the cancer cells. From the results it can be attributed that d’Bioimager can be used as a non-invasive imaging alternative to measure the morphological changes of the living cells in real time.

Citation Download Citation

Sreelatha Sarangapani, Rosmin Elsa Mohan, Ajeetkumar Patil, Matthew J. Lang, and Anand Asundi "3D/4D multiscale imaging in acute lymphoblastic leukemia cells: visualizing dynamics of cell death", Proc. SPIE 10449, Fifth International Conference on Optical and Photonics Engineering, 1044936 (13 June 2017); https://doi.org/10.1117/12.2270979

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