Paper
12 February 2018 Photodynamic inactivation using curcuminoids and Photogem on caenorhabditis elegans
Yulli R. Albuquerque, Sebastião Pratavieira, Vanderlei S. Bagnato, Natalia M. Inada, Larissa M. Souza, Ana Afonso, Clovis W. O. de Souza, Kleber T. Oliveira, Fernanda F. Anibal
Author Affiliations +
Abstract
Resistance to various anthelmintic drugs is reported in many animals and can become a severe problem for human and animal health. In this study, Photogem® and three curcuminoids compounds (curcumin, demethoxycurcumin, bisdemethoxycurcumin) were used as photosensitizers in the photodynamic inactivation (PDI) in the helminth model Caenorhabditis elegans to investigate the ability of this procedure to worm life cycle. Initially, the presence and location of the photosensitizers in the worm's body were verified by fluorescence confocal microscopy. Curcumin was deposited in the digestive tract and Photogem® along the body of the animal in the incubation time of 12 hours with the photosensitizer. Subsequently, a PDI procedure using a LED device was performed to illuminate the worms treated with the photosensitizers. The worms were observed by optical microscopy until 48 hours after the PDI to verify the changes in motility, the presence of eggs and larvae and the number of live worms. Curcuminoids tested separately and in combination and two light doses of 30 J/m2 no changes were observed in the life cycle of the worm at concentrations of 2 mM and 1 mM. However, in treatment with Photogem® and a light dose of 100 J/m2 a reduction in motility and reproduction of the worm with 0.2 mg/mL was observed after 6 hours of exposure, in addition to the death of most worms at concentrations of 6, 4, and 2 mg/mL. We suggest, therefore, that photodynamic inactivation with Photogem® may present an anthelmintic effect against C. elegans, but there is a need for studies on helminths with parasitic activity.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yulli R. Albuquerque, Sebastião Pratavieira, Vanderlei S. Bagnato, Natalia M. Inada, Larissa M. Souza, Ana Afonso, Clovis W. O. de Souza, Kleber T. Oliveira, and Fernanda F. Anibal "Photodynamic inactivation using curcuminoids and Photogem on caenorhabditis elegans", Proc. SPIE 10476, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII, 1047612 (12 February 2018); https://doi.org/10.1117/12.2283242
Lens.org Logo
CITATIONS
Cited by 1 scholarly publication.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Light emitting diodes

Luminescence

Molecules

Organisms

Confocal microscopy

Oxygen

Resistance

Back to Top