CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10487 > Article
Presentation
14 March 2018 Novel low level laser therapy enhanced vascular endothelial growth factor choroidal neovascularization in living rabbits monitored with multimodal photoacoustic, optical coherence tomography, and fluorescence imaging. (Conference Presentation)
Phuc Nguyen, Wei Zhang, Yanxiu Li, Yannis M. Paulus M.D.
Author Affiliations +
Proceedings Volume 10487, Multimodal Biomedical Imaging XIII; 1048706 (2018) https://doi.org/10.1117/12.2288564
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Choroidal neovascularization (CNV) from age-related macular degeneration (AMD) is the leading cause of permanent vision loss and blindness in adults in the developed world. Early detection of CNV results in improved visual outcomes in patients. The current study investigated the feasibility of low-level laser therapy (LLLT)-assisted vascular epithelial growth factor (VEGF) intravitreal injections to increase angiogenesis. In addition, the developed CNV model was evaluated by photoacoustic microscopy (PAM), optical coherence tomography (OCT), and fluorescein angiography (FA) imaging systems. Dual laser wavelengths of 660 and 780 nm were used to activate VEGF to enhance new blood vessels angiogenesis. Ten New Zealand white rabbits were injected intravitreally with recombinant human VEGF165 at a dose of 10 μg/0.1 mL injection followed by laser at an irradiance of 3 and 4 J/cm2 or control (no laser) New blood vessel formation was monitored by PAM, OCT, and FA. Area of neovascularization was imaged and quantified bi-weekly after treatment for 1 month. Laser-enhanced VEGF increased the area of neovascularization after treatment in comparison with VEGF injection only. The location of CNV was obviously identified by PAM, OCT, and FA. The proposed LLLT-assisted VEGF together with multimodal imaging system can serve as a useful technique for clinical detection and diagnostics of CNV and may provide the functional information in the changes.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Phuc Nguyen, Wei Zhang, Yanxiu Li, and Yannis M. Paulus M.D. "Novel low level laser therapy enhanced vascular endothelial growth factor choroidal neovascularization in living rabbits monitored with multimodal photoacoustic, optical coherence tomography, and fluorescence imaging. (Conference Presentation)", Proc. SPIE 10487, Multimodal Biomedical Imaging XIII, 1048706 (14 March 2018); https://doi.org/10.1117/12.2288564
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Optical coherence tomography
Laser therapeutics
Blood vessels
Imaging systems
Luminescence
Photoacoustic microscopy
Photoacoustic spectroscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top