Measuring in-vivo and in-situ ex-vivo the 3D deformation of the lamina cribrosa microstructure under elevated intraocular pressure

Junchao Wei; Bin Yang; Andrew P. Voorhees; Huong Tran; Bryn Brazile; Bo Wang; Joel Schuman; Matthew A. Smith; Gadi Wollstein; Ian A. Sigal

doi:10.1117/12.2291243

19 February 2018 Measuring in-vivo and in-situ ex-vivo the 3D deformation of the lamina cribrosa microstructure under elevated intraocular pressure

Junchao Wei, Bin Yang, Andrew P. Voorhees, Huong Tran, Bryn Brazile, Bo Wang, Joel Schuman, Matthew A. Smith, Gadi Wollstein, Ian A. Sigal

Author Affiliations +

Proceedings Volume 10496, Optical Elastography and Tissue Biomechanics V; 1049611 (2018) https://doi.org/10.1117/12.2291243
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Elevated intraocular pressure (IOP) deforms the lamina cribrosa (LC), a structure within the optic nerve head (ONH) in the back of the eye. Evidence suggests that these deformations trigger events that eventually cause irreversible blindness, and have therefore been studied in-vivo using optical coherence tomography (OCT), and ex-vivo using OCT and a diversity of techniques. To the best of our knowledge, there have been no in-situ ex-vivo studies of LC mechanics. Our goal was two-fold: to introduce a technique for measuring 3D LC deformations from OCT, and to determine whether deformations of the LC induced by elevated IOP differ between in-vivo and in-situ ex-vivo conditions. A healthy adult rhesus macaque monkey was anesthetized and IOP was controlled by inserting a 27- gauge needle into the anterior chamber of the eye. Spectral domain OCT was used to obtain volumetric scans of the ONH at normal and elevated IOPs. To improve the visibility of the LC microstructure the scans were first processed using a novel denoising technique. Zero-normalized cross-correlation was used to find paired corresponding locations between images. For each location pair, the components of the 3D strain tensor were determined using non-rigid image registration. A mild IOP elevation from 10 to 15mmHg caused LC effective strains as large as 3%, and about 50% larger in-vivo than in-situ ex-vivo. The deformations were highly heterogeneous, with substantial 3D components, suggesting that accurate measurement of LC microstructure deformation requires high-resolution volumes. This technique will help improve understanding of LC biomechanics and how IOP contributes to glaucoma.

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Junchao Wei, Bin Yang, Andrew P. Voorhees, Huong Tran, Bryn Brazile, Bo Wang, Joel Schuman, Matthew A. Smith, Gadi Wollstein, and Ian A. Sigal "Measuring in-vivo and in-situ ex-vivo the 3D deformation of the lamina cribrosa microstructure under elevated intraocular pressure", Proc. SPIE 10496, Optical Elastography and Tissue Biomechanics V, 1049611 (19 February 2018); https://doi.org/10.1117/12.2291243

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