Two-photon FLIM imaging and femtosecond laser nano processing of IPS cells and stem cells (Conference Presentation)

Karsten König; Aisada Uchugonova; Hans Georg Breunig; Ana Batista

doi:10.1117/12.2287125

14 March 2018 Two-photon FLIM imaging and femtosecond laser nano processing of IPS cells and stem cells (Conference Presentation)

Karsten König, Aisada Uchugonova, Hans Georg Breunig, Ana Batista

Author Affiliations +

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104980P (2018) https://doi.org/10.1117/12.2287125
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Stem cells and 'induced pluripotent stem cells' (iPS cells) attract considerable attention in medicine. So far, iPS cells which morphologically and functionally resemble embryonic stem cells have been generated by means of viruses through the reprogramming of somatic cells for research purposes. Typically, optical techniques such as light microscopy in combination with fluorescent markers are applied, sometimes accompanied with fluorescence-activated cell sorting (FACS), for the characterization and isolation of both tissue-specific stem and iPS cells. Nowadays, modern laser-based techniques such as nonlinear multiphoton microscopy and optical transfection using femtosecond lasers are used for iPS and stem cell imaging as well as for virus-free optical reprogramming.

Conference Presentation

Citation Download Citation

Karsten König, Aisada Uchugonova, Hans Georg Breunig, and Ana Batista "Two-photon FLIM imaging and femtosecond laser nano processing of IPS cells and stem cells (Conference Presentation)", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104980P (14 March 2018); https://doi.org/10.1117/12.2287125

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available