Integrated SRS and fluorescence imaging for study of thermogenesis and lipid metabolism in vivo

Sicong He; Yitai An; Xuesong Li; Zhenguo Wu; Jianan Y. Qu

doi:10.1117/12.2289659

23 February 2018 Integrated SRS and fluorescence imaging for study of thermogenesis and lipid metabolism in vivo

Sicong He, Yitai An, Xuesong Li, Zhenguo Wu, Jianan Y. Qu

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104981I (2018) https://doi.org/10.1117/12.2289659
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

In this work, we developed a label-free imaging and spectroscopy method to assess the metabolism and thermogenesis of mouse adipose tissues in vivo. An optical redox ratio based on the endogenous fluorescence of mitochondrial coenzymes was used as a biomarker to determine the metabolic state of adipocytes during thermogenesis. The morphological and functional characteristics of different types of adipocytes were assessed in vivo and their thermogenic activities were monitored in real time with a robust spectroscope system.

Conference Presentation

Citation Download Citation

Sicong He, Yitai An, Xuesong Li, Zhenguo Wu, and Jianan Y. Qu "Integrated SRS and fluorescence imaging for study of thermogenesis and lipid metabolism in vivo", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981I (23 February 2018); https://doi.org/10.1117/12.2289659

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