High-density single particle tracking in thick media with depth selectivity (Conference Presentation)

Roni Shaashoua; Alberto Bilenca

doi:10.1117/12.2288892

14 March 2018 High-density single particle tracking in thick media with depth selectivity (Conference Presentation)

Roni Shaashoua, Alberto Bilenca

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104990Y (2018) https://doi.org/10.1117/12.2288892
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Single fluorescent particle tracking (SPT) is a technique often used in the biophysical sciences in order to explore the environment of soft matter [C Manzo et al, Rep Prog Phys 78, 124601 (2015); H Shen et al, Chem Rev 117, 7331–7376 (2017)]. SPT has typically been performed using widefield sample illumination and fluorescent spheres at low concentration, thus limiting the achievable tracking depth and the density of mapped particle trajectories within the sample (and hence the statistical accuracy with which particle motions can be analyzed). In this talk, we propose to use a light sheet fluorescence microscope with photoactivatable carriers for high density SPT in thick samples with depth selectivity. In particular, we will demonstrate the usefulness of this approach for investigating the environments of heterogeneous soft materials, such as agarose gels, with improved statistical accuracy, and for providing precise depth information on the mechanical and dynamical properties of inhomogeneous soft matter. Furthermore, a discussion on the applicability of our method to probe material rheology at the nanometer-scale will be presented.

Conference Presentation

Citation Download Citation

Roni Shaashoua and Alberto Bilenca "High-density single particle tracking in thick media with depth selectivity (Conference Presentation)", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104990Y (14 March 2018); https://doi.org/10.1117/12.2288892

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available