Hyper-spectrum scanning laser optical tomography

Lingling Chen; Guiye Li; Yingchao Li; Lina Liu; Ang Liu; Xuejuan Hu; Shuangchen Ruan

doi:10.1117/12.2287062

23 February 2018 Hyper-spectrum scanning laser optical tomography

Lingling Chen, Guiye Li, Yingchao Li, Lina Liu, Ang Liu, Xuejuan Hu, Shuangchen Ruan

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104991L (2018) https://doi.org/10.1117/12.2287062
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We describe a quantitative fluorescence projection tomography technique which measures the three-dimensional fluorescence spectrum in biomedical samples with size up to several millimeters. This is achieved by acquiring a series of hyperspectral images, by using laser scanning scheme, at different projection angles. We demonstrate that this technique provide a quantitative measure of the fluorescence signal by comparing the spectrum and intensity profile of a fluorescent bead phantom and also demonstrate its application to differentiating the extrinsic label and the autofluorescence in a mouse embryo.

Citation Download Citation

Lingling Chen, Guiye Li, Yingchao Li, Lina Liu, Ang Liu, Xuejuan Hu, and Shuangchen Ruan "Hyper-spectrum scanning laser optical tomography", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991L (23 February 2018); https://doi.org/10.1117/12.2287062

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