Estimation of the effect of radionuclide contamination on Vicia sativa L. induction of chlorophyll fluorescence parameters using "Floratest" optical biosensor

Yu. Ruban; V. Illienko; N. Nesterova; O. Pareniuk; K. Shavanova

doi:10.1117/12.2297563

7 December 2017 Estimation of the effect of radionuclide contamination on Vicia sativa L. induction of chlorophyll fluorescence parameters using "Floratest" optical biosensor

Yu. Ruban, V. Illienko, N. Nesterova, O. Pareniuk, K. Shavanova

Author Affiliations +

Proceedings Volume 10592, Biophotonics—Riga 2017; 105920M (2017) https://doi.org/10.1117/12.2297563
Event: The Second International Conference "Biophotonics-Riga 2017", 2017, Riga, Latvia

Abstract

The presented research was aimed to determine the parameters of chlorophyll fluorescence (IChH) curve induction for Vicia sativa L. that were grown on radionuclide contaminated soils by using "Floratest" fluorometer. Plants were inoculated with 5 species of bacteria that might potentially block radionuclide uptake (Agrobacterium radiobacter IМВВ-7246, Azotobacter chroococcum UКМВ-6082, A. chroococcum UКМВ-6003, Bacillus megaterium UКМВ-5724, Rhizobium leguminosarum bv. viceae) and grown in sod-podzolic, сhernozem and peat-bog soils, contaminated with 137Cs (4000±340 Bq/kg). As a result of research, it was determined that the most stressful factors for vetch plants are combination of soil radionuclide and presence of Bacillus megaterium UКМ В-5724, as the number of inactive chlorophyll increased. In addition, the vetch plants significantly increased fixed level of fluorescence (Fst) under the influence of radioactive contamination in presence of Bacillus megaterium UKM B-5724, indicating inhibition of photosynthetic reactions. Other bacteria showed radioprotective properties in almost all types of soil.

Citation Download Citation

Yu. Ruban, V. Illienko, N. Nesterova, O. Pareniuk, and K. Shavanova "Estimation of the effect of radionuclide contamination on Vicia sativa L. induction of chlorophyll fluorescence parameters using "Floratest" optical biosensor", Proc. SPIE 10592, Biophotonics—Riga 2017, 105920M (7 December 2017); https://doi.org/10.1117/12.2297563

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available