Rapid vertical tissue imaging with clinical multiphoton tomography

Hans Georg Breunig; Benjamin Sauer; Ana Batista; Karsten König

doi:10.1117/12.2323441

24 May 2018 Rapid vertical tissue imaging with clinical multiphoton tomography

Hans Georg Breunig, Benjamin Sauer, Ana Batista, Karsten König

Proceedings Volume 10679, Optics, Photonics, and Digital Technologies for Imaging Applications V; 106790N (2018) https://doi.org/10.1117/12.2323441
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Multiphoton imaging commonly relies on laser-scanning setups which quickly image horizontal sections (x y images) by pixelwise scanning a sample region with focused laser pulses. Different horizontal planes are imaged by adjusting the distance relative between focusing optics and sample. However, in many cases actually a visualization of vertical sections is desired that then can only be obtained indirectly from time-consuming acquisition and processing of complete volume scans. We present a modified multiphoton tomograph for clinical in vivo and ex vivo tissue imaging with direct and fast x-z-imaging capability and exemplify different applications spanning from visualizing anatomic structures to substance penetration studies. The fast x-z imaging is realized by synchronizing the scanning-mirror movement with the tuning of the relative distance between sample and focusing optics

Citation Download Citation

Hans Georg Breunig, Benjamin Sauer, Ana Batista, and Karsten König "Rapid vertical tissue imaging with clinical multiphoton tomography", Proc. SPIE 10679, Optics, Photonics, and Digital Technologies for Imaging Applications V, 106790N (24 May 2018); https://doi.org/10.1117/12.2323441

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