Multi-dimensional digital holographic microscopy

Osamu Matoba; Xiangyu Quan; Manoj Kumar; Yasuhiro Awatsuji

doi:10.1117/12.2501202

5 November 2018 Multi-dimensional digital holographic microscopy

Osamu Matoba, Xiangyu Quan, Manoj Kumar, Yasuhiro Awatsuji

Proceedings Volume 10815, Optical Design and Testing VIII; 108150T (2018) https://doi.org/10.1117/12.2501202
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.

Citation Download Citation

Osamu Matoba, Xiangyu Quan, Manoj Kumar, and Yasuhiro Awatsuji "Multi-dimensional digital holographic microscopy ", Proc. SPIE 10815, Optical Design and Testing VIII, 108150T (5 November 2018); https://doi.org/10.1117/12.2501202

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