Multimodal imaging with optical coherence tomography and multiphoton microscopy of human hip joint osteoarthritis

Xin Zhou; Hanadi Alousaimi; Ruochen Wen; Lin Huang; Rizhi Wang; Shuo Tang

doi:10.1117/12.2511012

26 February 2019 Multimodal imaging with optical coherence tomography and multiphoton microscopy of human hip joint osteoarthritis

Xin Zhou, Hanadi Alousaimi, Ruochen Wen, Lin Huang, Rizhi Wang, Shuo Tang

Author Affiliations +

Proceedings Volume 10868, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII; 1086809 (2019) https://doi.org/10.1117/12.2511012
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Osteoarthritis (OA) is the most common form of arthritis, where the protective cartilage on the ends of bones wears down over time, causing pain, tenderness, stiffness, loss of flexibility and bone spurs. Degenerative alterations start before cartilage loss happens, which include surface swelling, cartilage fibrillation, and calcification. Detecting the early degenerative alterations can assist the diagnosis of early-stage OA. In this study, two imaging modalities are applied on human hip-joint specimens in ex vivo imaging, including polarization-sensitive optical coherence tomography (PS-OCT) and multiphoton microscopy (MPM). OCT detects the layered tissue structure of cartilage and bone using backscattered light and PS-OCT is a functional extension of OCT. PS-OCT measures tissue birefringence which is sensitive to the orderly organization of collagen in cartilage. MPM can visualize collagen fibers with sub-cellular resolution. Complementary information about cartilage on the cellular and tissue level can be obtained by the multimodal imaging. Using the multimodal system, the variation of the thickness of the cartilage structural zones, abnormal birefringence caused by collagen alterations and fibrillation, and uneven structure resulted from calcification are imaged and quantified. The imaging results show distinctive features of degenerative alterations in the OA specimen, such as uneven tissue surface, fibrillation, and reduced birefringence. It is shown that PS-OCT has great potential in detecting early stage OA.

Conference Presentation

Citation Download Citation

Xin Zhou, Hanadi Alousaimi, Ruochen Wen, Lin Huang, Rizhi Wang, and Shuo Tang "Multimodal imaging with optical coherence tomography and multiphoton microscopy of human hip joint osteoarthritis", Proc. SPIE 10868, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII, 1086809 (26 February 2019); https://doi.org/10.1117/12.2511012

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available