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4 March 2019 Wide-field quantitative phase imaging and diffraction tomography with Fourier ptychography (Conference Presentation)
Chao Zuo, Jiaji Li
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Proceedings Volume 10887, Quantitative Phase Imaging V; 1088709 (2019) https://doi.org/10.1117/12.2513257
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
High-throughput quantitative phase imaging (QPI) is essential to cellular phenotypes characterization as it allows high-content cell analysis and avoids adverse effects of staining reagents on cellular viability and cell signaling. Among different approaches, Fourier ptychographic microscopy (FPM) is probably the most promising technique to realize high-throughput QPI by synthesizing a wide-field, high-resolution complex image from multiple angle-variably illuminated, low-resolution images. However, the large dataset requirement in conventional FPM significantly limits its imaging speed, resulting in low temporal throughput. In this talk, we report two optimum illumination schemes for FPM to achieve high-speed or even single-shot QPI. We present the high-speed imaging results of in vitro Hela cells mitosis and apoptosis at a frame rate of 25 Hz with a full-pitch resolution of 655 nm at a wavelength of 525 nm (effective NA = 0.8) across a wide field-of-view (FOV) of 1.77 mm2, corresponding to a space–bandwidth–time product of 411 megapixels per second. We also discuss how FPM can be extended to optical diffraction tomography (ODT) under Born or Rytov approximation, achieving super and depth resolved 3D imaging over a wide FOV.
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Chao Zuo and Jiaji Li "Wide-field quantitative phase imaging and diffraction tomography with Fourier ptychography (Conference Presentation)", Proc. SPIE 10887, Quantitative Phase Imaging V, 1088709 (4 March 2019); https://doi.org/10.1117/12.2513257
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KEYWORDS
Diffraction
Phase imaging
Tomography
Cell death
High speed imaging
Image resolution
In vitro testing
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