Photodynamic inactivation of Candida albicans using a synthesized bacteriochlorin as a photosensitizer

Laura Cristina Angioletto Martins; Thaila Quatrini Corrêa; Sebastião Pratavieira; Marciana P. Uliana; Kleber Thiago de Oliveira; Vanderlei Salvador Bagnato; Clovis Wesley Oliveira de Souza

doi:10.1117/12.2526403

7 August 2019 Photodynamic inactivation of Candida albicans using a synthesized bacteriochlorin as a photosensitizer

Laura Cristina Angioletto Martins, Thaila Quatrini Corrêa, Sebastião Pratavieira, Marciana P. Uliana, Kleber Thiago de Oliveira, Vanderlei Salvador Bagnato, Clovis Wesley Oliveira de Souza

Author Affiliations +

Proceedings Volume 11070, 17th International Photodynamic Association World Congress; 110708K (2019) https://doi.org/10.1117/12.2526403
Event: 17th International Photodynamic Association World Congress, 2019, Cambridge, Massachusetts, United States

Abstract

In this study, Candida albicans in its planktonic form, was used as target microorganism. This species frequently appears in superficial and invasive infections because of its pathogenic potential, and it is becoming highly resistant. Bacteriochlorin was used as a photosensitizer, presenting absorption around 780 nm and low toxicity in the absence of light. The objective was to evaluate its photodynamic inactivation potential in Candida albicans. The photosensitizer was synthesized from the extraction of bacteriochlorophylls derived from non-sulfurous purple bacteria and the converted to bacteriochlorin. The inoculum of Candida albicans was grown for 24 hours and adjusted to the concentration of 106 CFU/mL. The device used for the emission of light was a homemade device with LEDs of 780 nm wavelength. The quantitative evaluation of viable cells was performed by spread plate in Sabouraud Dextrose Agar. The results showed that this new bacteriochlorin is not much stable in its absorption peak, being necessary a better chemical characterization to verify its antimicrobial potential.

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Laura Cristina Angioletto Martins, Thaila Quatrini Corrêa, Sebastião Pratavieira, Marciana P. Uliana, Kleber Thiago de Oliveira, Vanderlei Salvador Bagnato, and Clovis Wesley Oliveira de Souza "Photodynamic inactivation of Candida albicans using a synthesized bacteriochlorin as a photosensitizer", Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 110708K (7 August 2019); https://doi.org/10.1117/12.2526403

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