Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue

Laura M. G. van Huizen; Daniëlle Seinstra; Chris Dickhoff; Teodora Radonic; Idris Bahce; Frank van Mourik; Jouke T. Annema; Johannes M. A. Daniels; Wim-Jan P. van Boven; Marie Louise Groot

doi:10.1117/12.2527899

22 July 2019 Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue

Laura M. G. van Huizen, Daniëlle Seinstra, Chris Dickhoff, Teodora Radonic, Idris Bahce, Frank van Mourik, Jouke T. Annema, Johannes M. A. Daniels, Wim-Jan P. van Boven, Marie Louise Groot

Author Affiliations +

Proceedings Volume 11079, Medical Laser Applications and Laser-Tissue Interactions IX; 110790J (2019) https://doi.org/10.1117/12.2527899
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Third and second harmonic generation (THG/SHG) microscopy is a novel imaging technique that has been suggested as a promising clinical tool, mainly for cancer diagnosis. Here, we test for the first time a compact, transportable ex-vivo THG/SHG microscope (Tritos Diagnostics B.V.) for clinical use. In lung surgery, immediate feedback on the nature of the excised tissue is important. We will present the first results of an ongoing study in which we image both lung tumor tissue immediately after excision and frozen section biopsies, and compare the images to the golden standard histology. Prior to this study, we performed a set of experiments collecting 3D images of mouse lung tissue to show that the combination of THG and SHG microscopy reveals key lung morphology components.

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Laura M. G. van Huizen, Daniëlle Seinstra, Chris Dickhoff, Teodora Radonic, Idris Bahce, Frank van Mourik, Jouke T. Annema, Johannes M. A. Daniels, Wim-Jan P. van Boven, and Marie Louise Groot "Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue", Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 110790J (22 July 2019); https://doi.org/10.1117/12.2527899

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