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The single-beam gradient force optical tweezers have transformed various fields of scientific research by enabling manipulation and characterization of single molecules. Conventional optical tweezers pose limitations in trapping particles in the sub-Rayleigh regime. These limitations have been overcome with the help of plasmonic nanoapertures like the double-nanohole aperture. A modified colloidal lithography technique has been used in fabrication of double-nanohole apertures achieving dimensions appropriate for trapping single molecules in this regime. This paper demonstrates optical trapping of a single 10 nm enzyme, rubisco, using double-nanohole apertures fabricated using the modified colloidal lithography technique as well as presents the results from transmission characterization of different double-nanohole apertures carried out using the finite-difference time-domain (FDTD) simulations.
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