Detection of singlet oxygen luminescence in skin phantom based on optical fiber detection

Ying Hu; Lisheng Lin; Lina Liu; Yunfei Yu; Xianglian Liao; Ying Gu; Buhong Li

doi:10.1117/12.2537692

20 November 2019 Detection of singlet oxygen luminescence in skin phantom based on optical fiber detection

Ying Hu, Lisheng Lin, Lina Liu, Yunfei Yu, Xianglian Liao, Ying Gu, Buhong Li

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111902X (2019) https://doi.org/10.1117/12.2537692
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Singlet oxygen (¹O₂) is widely considered as the major cytotoxic species generated during Type-II photochemical reaction of photodynamic therapy (PDT), and its production is crucial for the treatment outcome of PDT. However, it is still a challenging task for direct detection of ¹O₂ by using an optical system as its extremely weak luminescence at 1270 nm. In previous study, a high-sensitivity optical fiber detection system has been developed to measure the time-resolved ¹O₂ luminescence spectra. In this study, an optical phantom of skin tissue has been built to simulate the skin optical properties for research in photodynamic therapy (PDT). The phantom consists of an absorber (ink) and a scatterer (Intralipid) and phosphate buffer saline (PBS). Rose Bengal (RB) was utilized as the model photosensitizer to generate ¹O₂. The time-resolved ¹O₂ luminescence spectroscopy were measured by using a ¹O₂ luminescence detection system with a fiber prober in tissue phantom. Furthermore, the effect of absorption coefficient (μ_a) and scattering coefficient (μ_s) on the photosensitizer triple state lifetime (τ_T) and ¹O₂ luminescence lifetime (τ_D) was investigated as well. The results indicated that the integrated intensity of ¹O₂ luminescence decrease with the increase of μ_a and μ_s. μ_a has no significant effect on τ_T and τ_D. τ_T increase with the increase of μ_s, and it finally stabilized around 1.6 μs. Meanwhile, τ_D decrease firstly and then increase slowly, and it finally stabilized around 14 μs. This result indicates that this system for ¹O₂ luminescence have a potential for clinical applications in PDT dosimetry.

Citation Download Citation

Ying Hu, Lisheng Lin, Lina Liu, Yunfei Yu, Xianglian Liao, Ying Gu, and Buhong Li "Detection of singlet oxygen luminescence in skin phantom based on optical fiber detection", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902X (20 November 2019); https://doi.org/10.1117/12.2537692

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KEYWORDS

Luminescence

Oxygen

Scattering

Optical fibers

Skin

Absorption

Photodynamic therapy

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