Estimation of the collagen and elastin condition at lymphedema using multiphoton microscopy

Viktor V. Nikolaev; Ekaterina A. Sandykova; Oksana S. Kurochkina; Denis A. Vrazhnov; Natalia A. Krivova; Yury V. Kistenev; Elena S. Sim

doi:10.1117/12.2540604

18 December 2019 Estimation of the collagen and elastin condition at lymphedema using multiphoton microscopy

Viktor V. Nikolaev, Ekaterina A. Sandykova, Oksana S. Kurochkina, Denis A. Vrazhnov, Natalia A. Krivova, Yury V. Kistenev, Elena S. Sim

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Proceedings Volume 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics; 112080E (2019) https://doi.org/10.1117/12.2540604
Event: XXV International Symposium, Atmospheric and Ocean Optics, Atmospheric Physics, 2019, Novosibirsk, Russian Federation

Abstract

Methods of two-photon microscopy are widely used in the study of biological objects, in particular, skin, due to the possibility to study objects both on the surface and at depth without attracting additional fluorophores due to endogenous autofluorescence. In this paper, the methods of image analysis of the AF signal and SHG signal are applied to assess the condition of the skin during the development of lymphedema. It is shown that for groups of healthy tissue and lymphedematous using SAAID distribution histograms, changes in tissues can be detected.

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Viktor V. Nikolaev, Ekaterina A. Sandykova, Oksana S. Kurochkina, Denis A. Vrazhnov, Natalia A. Krivova, Yury V. Kistenev, and Elena S. Sim "Estimation of the collagen and elastin condition at lymphedema using multiphoton microscopy", Proc. SPIE 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics, 112080E (18 December 2019); https://doi.org/10.1117/12.2540604

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KEYWORDS

Tissues

Collagen

Second-harmonic generation

Skin

Multiphoton microscopy

Photons

Fluorescence lifetime imaging

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