Field-portable multi-modal chip-based fluorescence, bright field and quantitative phase microscopy using smartphone detecting system

Dalip Singh Mehta; Shilpa Tayal; Anuj Saxena; Veena Singh; Vishesh Kumar Dubey

doi:10.1117/12.2546258

21 February 2020 Field-portable multi-modal chip-based fluorescence, bright field and quantitative phase microscopy using smartphone detecting system

Dalip Singh Mehta, Shilpa Tayal, Anuj Saxena, Veena Singh, Vishesh Kumar Dubey

Author Affiliations +

Proceedings Volume 11230, Optics and Biophotonics in Low-Resource Settings VI; 112300A (2020) https://doi.org/10.1117/12.2546258
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We report the development of field-portable multi-modal chip-based fluorescence, bright field and quantitative phase microscopy using smartphone detecting system. Fluorescence microscopy provide molecular information of the specimen with excellent specificity, while phase microscopy provides quantitative information of the specimen. Quantifying the optical phase shifts associated with biological structures gives access to information about morphology and dynamics at the nanometer scale. Here, we propose an integrated waveguide chip-based total internal reflection fluorescence (TIRF) microscopy and quantitative phase microscopy (QPM). We have developed microLED with cylindrical beam profile to couple excitation light into the edges of glass slide easily and efficiently. The evanescent field present on top of a waveguide surface is used to excite the fluorescence and a mobile phone microscope is used to collect the signal. Waveguide chip-based TIRF microscopy benefits from decoupling of illumination and collection light path, large field of view imaging and pre-aligned configuration for multi-color TIRF imaging. Light for bright field imaging and QPM integrated in the transmission mode. A microscope objective is used for collecting the fluorescence excited by evanescent field and transmitted light for bright field and quantitative phase microscopy (QPM). A compact and common path interferometer is used for QPM. The entire device is fabricated using three-D printer and integrated into one, which is compact and field portable. Images are recorded using a smart phone. Experimental results of onion epithelial cells, polystyrene microspheres and normal breast tissue are presented. The cost of entire system is very less.

Conference Presentation

Citation Download Citation

Dalip Singh Mehta, Shilpa Tayal, Anuj Saxena, Veena Singh, and Vishesh Kumar Dubey "Field-portable multi-modal chip-based fluorescence, bright field and quantitative phase microscopy using smartphone detecting system", Proc. SPIE 11230, Optics and Biophotonics in Low-Resource Settings VI, 112300A (21 February 2020); https://doi.org/10.1117/12.2546258

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