Characterisation of a Bessel beam optical cell sorting system using microspheres

Masixole Yvonne Lugongolo; Saturnin Ombinda-Lemboumba; Sello Manoto; Patience Mthunzi-Kufa

doi:10.1117/12.2541971

20 February 2020 Characterisation of a Bessel beam optical cell sorting system using microspheres

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Sello Manoto, Patience Mthunzi-Kufa

Proceedings Volume 11238, Optical Interactions with Tissue and Cells XXXI; 1123804 (2020) https://doi.org/10.1117/12.2541971
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Accurate sorting of specific particles in a mixed population is a desirable capability in the field of biomedical sciences. This enables researchers to purify samples by selecting only the particles of interest. Optical sorting is achieved by using a Bessel beam, which is a non-diffracting, propagation invariant light pattern consisting of concentric rings around a bright central core. This type of beam profile has the ability to employ optical forces in manipulating matter in a sterile environment without physical interaction. The concentric rings enable the simultaneous manipulation of particles of various characteristics in multiple planes due to the different power intensity distributions. Sorting with Bessel beam is an attractive approach using small sample volumes (microliter ranges), which becomes beneficial when working with rare particles of interest and in small samples. In this study a home built Bessel beam optical sorting setup was used to sort polystyrene and silica microspheres of different sizes and refractive indices. Our preliminary results showed that the polystyrene microspheres travelled quicker than the silica type of spheres with the same size due to the high refractive indices. These findings indicate the potential application of sorting different cells with varying refractive indices such as differentiating HIV infected cells from uninfected cells.

Citation Download Citation

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Sello Manoto, and Patience Mthunzi-Kufa "Characterisation of a Bessel beam optical cell sorting system using microspheres", Proc. SPIE 11238, Optical Interactions with Tissue and Cells XXXI, 1123804 (20 February 2020); https://doi.org/10.1117/12.2541971

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KEYWORDS

Bessel beams

Refractive index

Particles

Optical sorting

Silica

Mirrors

Beam propagation method

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