In vivo nano-scale vibrometry in apical-basal ends of contractile outer hair cells in the mammalian cochlea by supercontinuum source spectral-domain OCT

Fumiaki Nin; Samuel Choi; Takeru Ota; Hiroshi Hibino

doi:10.1117/12.2539303

21 February 2020 In vivo nano-scale vibrometry in apical-basal ends of contractile outer hair cells in the mammalian cochlea by supercontinuum source spectral-domain OCT

Fumiaki Nin, Samuel Choi, Takeru Ota, Hiroshi Hibino

Author Affiliations +

Proceedings Volume 11239, Dynamics and Fluctuations in Biomedical Photonics XVII; 112390B (2020) https://doi.org/10.1117/12.2539303
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The cochlea of the inner ear transduces sound energy into electrical signals that are essential for audition. This transduction is processed in nano-scale vibration of the cochlear sensory epithelia. In mammals, the epithelia contain various cells and structures: inner hair cells, outer hair cells (OHCs), Deiters’ cells, basilar membrane, reticular lamina, etc. The sound elicits vibration in all these constituents. Among them, only OHCs cell body actively and periodically changes in length in association with the vibration. The unique mechanical activity of OHCs modifies the sound elicited vibration in the epithelia with a feedback mechanism. Although the modification is considered to critically contribute to the high sensitivity and sharp tuning in hearing through sensory IHCs, the real motion of OHCs remains uncertain. Vibrometrical studies of cochlear mechanics has revealed important vibration of the cell bodies involving the epithelia. However, difference in vibration pattern of the apical and basal ends of the cell has remain uncertain due to low spatial resolution of the system and low reflectivity of the cells. We performed a spectral domain OCT (SD-OCT) vibrometry by using the modified commercial SD-OCT system. Because the broad spectral bandwidth and strong power of the light source improve a performance of OCT systems in both of imaging and vibrometry, we introduced a supercontinuum light source into the commercial system. Our system achieved cellular-level tomographic imaging and subnano-scale vibration measurement in the transparent epithelia with the recording time of 100 ms in in vivo animal.

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Citation Download Citation

Fumiaki Nin, Samuel Choi, Takeru Ota, and Hiroshi Hibino "In vivo nano-scale vibrometry in apical-basal ends of contractile outer hair cells in the mammalian cochlea by supercontinuum source spectral-domain OCT", Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390B (21 February 2020); https://doi.org/10.1117/12.2539303

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