Time-resolved multi-dimensional fluorescence imaging using a Digital-Micromirror-Device and a SPAD-array detector

A. Farina; L. Di Sieno; G. Acconcia; A. Gulinatti; G. Valentini; I. Rech; C. D'Andrea

doi:10.1117/12.2546881

24 February 2020 Time-resolved multi-dimensional fluorescence imaging using a Digital-Micromirror-Device and a SPAD-array detector

A. Farina, L. Di Sieno, G. Acconcia, A. Gulinatti, G. Valentini, I. Rech, C. D'Andrea

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430N (2020) https://doi.org/10.1117/12.2546881
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

A widefield system for multidimensional fluorescence imaging capable of resolving space, time and wavelength is developed and validated on a synthetic fluorescence sample. The system enables structured illumination and compressing detection. A compression strategy based on an a-priori information obtained by a camera is validated and proved to be effective at compression ratio of about 90%.

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A. Farina, L. Di Sieno, G. Acconcia, A. Gulinatti, G. Valentini, I. Rech, and C. D'Andrea "Time-resolved multi-dimensional fluorescence imaging using a Digital-Micromirror-Device and a SPAD-array detector", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430N (24 February 2020); https://doi.org/10.1117/12.2546881

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