Advanced volumetric light field microscopy with temporal-focusing multiphoton selective excitation (Conference Presentation)

Feng-Chun Hsu; Yong Da Sie; Chia-Yuan Chang; Chun-Yu Lin; Yvonne Y. Hu; Shean-Jen Chen

doi:10.1117/12.2546629

9 March 2020 Advanced volumetric light field microscopy with temporal-focusing multiphoton selective excitation (Conference Presentation)

Feng-Chun Hsu, Yong Da Sie, Chia-Yuan Chang, Chun-Yu Lin, Yvonne Y. Hu, Shean-Jen Chen

Author Affiliations +

Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 112450G (2020) https://doi.org/10.1117/12.2546629
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

In this study, we implement temporal-focusing multiphoton selective excitation (TFMPSE) to light field microscopy (LFM), illuminating only the volume of interest, thus significantly reducing the background noise and providing higher contrast and accuracy for the light field image reconstruction; furthermore, offering higher penetration depth in scattering tissue via multiphoton. 3D human-skin in situ immunofluorescence images are used to demonstrate volumetric bioimaging capability. The volume rate of the TFMPSE-LFM can achieve around 100 volumes per second

Conference Presentation

Citation Download Citation

Feng-Chun Hsu, Yong Da Sie, Chia-Yuan Chang, Chun-Yu Lin, Yvonne Y. Hu, and Shean-Jen Chen "Advanced volumetric light field microscopy with temporal-focusing multiphoton selective excitation (Conference Presentation)", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450G (9 March 2020); https://doi.org/10.1117/12.2546629

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