Quantitative infrared photothermal microscopy

Ilia M. Pavlovetc; Eduard A. Podshivaylov; Pavel A. Frantsuzov; Gregory V. Hartland; Masaru Kuno

doi:10.1117/12.2545159

13 February 2020 Quantitative infrared photothermal microscopy

Ilia M. Pavlovetc, Eduard A. Podshivaylov, Pavel A. Frantsuzov, Gregory V. Hartland, Masaru Kuno

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 1124613 (2020) https://doi.org/10.1117/12.2545159
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Infrared photothermal heterodyne imaging (IR-PHI) represents a convenient, table top approach for conducting super-resolution imaging and spectroscopy throughout the all-important mid infrared (MIR) spectral region. Although IR-PHI provides label-free, super-resolution MIR absorption information, it is not quantitative. In this study, we establish quantitative relationships between observed IR-PHI signals and relevant photothermal parameters of investigated specimens. Specifically, we conduct a size series analysis of different radii polystyrene (PS) beads to quantitatively link IR-PHI signal contrast to specimen heat capacity, thermo-optic coefficient, MIR peak absorption cross-section, and scattering cross-section at IR-PHI’s probe wavelength.

Citation Download Citation

Ilia M. Pavlovetc, Eduard A. Podshivaylov, Pavel A. Frantsuzov, Gregory V. Hartland, and Masaru Kuno "Quantitative infrared photothermal microscopy", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124613 (13 February 2020); https://doi.org/10.1117/12.2545159

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