Differential sensing technique for correlation-based adaptive optics

Hayao Kikuchi; Yusuke Honma; Noriaki Miura; Takatoshi Shibuya; Yosuke Tamada; Atsushi Matsuda; Masayuki Hattori

doi:10.1117/12.2545717

17 February 2020 Differential sensing technique for correlation-based adaptive optics

Hayao Kikuchi, Yusuke Honma, Noriaki Miura, Takatoshi Shibuya, Yosuke Tamada, Atsushi Matsuda, Masayuki Hattori

Proceedings Volume 11248, Adaptive Optics and Wavefront Control for Biological Systems VI; 1124811 (2020) https://doi.org/10.1117/12.2545717
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We have developed a microscopic adaptive optics (AO) system that corrects wavefront phase errors induced by complex structures of biological samples. The technique of correlation-based Shack-Hartmann (SH) sensing used in the AO system enables wavefront measurement using complex structures in a target as the reference. However, sub-images in the SH sensor become deformed dependently on the positions of sub-apertures as the NA of the microscopic objective is higher. This often deteriorates the accuracy of wavefront sensing. To mitigate the undesirable effect, we here propose a differential wavefront sensing technique with a mathematical formula, which is expected to measure wavefront at a better precision. Because differences in image shapes are less significant between nearby SAs, correlations between adjacent SAs are measured in the proposed method. We confirmed that the AO system worked as designed by experiments.

Citation Download Citation

Hayao Kikuchi, Yusuke Honma, Noriaki Miura, Takatoshi Shibuya, Yosuke Tamada, Atsushi Matsuda, and Masayuki Hattori "Differential sensing technique for correlation-based adaptive optics", Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124811 (17 February 2020); https://doi.org/10.1117/12.2545717

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